Evidence map›Paper›PMID 38281217›Full record

ArticleMolecular biology reports2024

Integrated scRNAseq analyses of mouse cochlear supporting cells reveal the involvement of Ezh2 in hair cell regeneration.

Yifan Zhao, Ze Xu, Ran Zhang, Mingli Liu, Chengcheng Lu, Chunxin Fan, Jian Wang

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Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Yifan ZhaoInternational Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, China.
Ze XuInternational Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, China.
Ran ZhangInternational Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, China.
Mingli LiuKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China.
Chengcheng LuInternational Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, China.
Chunxin FanInternational Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, China. cxfan@shou.edu.cn.
Jian WangInternational Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, China. j_wang@shou.edu.cn.ORCID http://orcid.org/0000-0001-8699-4998
Shanghai Ocean University · CNShanghai Genon Biological Products (China) · CNShougang (China) · CN

Funding

National Natural Science Foundation of China 31702329National Natural Science Foundation of China 31772406
6 · The paper itself

Abstract

backgroundIn lower vertebrates like fish, the inner ear and lateral line hair cells (HCs) can regenerate after being damaged by proliferation/differentiation of supporting cells (SCs). However, the HCs of mouse cochlear could only regenerate within one to two weeks after birth but not for adults. METHODS AND

resultsTo better understand the molecular foundations, we collected several public single-cell RNA sequencing (scRNAseq) data of mouse cochleae from E14 to P33 and extracted the prosensory and supporting cells specifically. Gene Set Enrichment Analysis (GSEA) results revealed a down-regulation of genes in Notch signaling pathway during postnatal stages (P7 and P33). We also identified 107 time-course co-expression genes correlated with developmental stage and predicated that EZH2 and KLF15 may be the key transcriptional regulators for these genes. Expressions of candidate target genes of EZH2 and KLF15 were also found in supporting cells of the auditory epithelia in chick and the neuromasts in zebrafish. Furthermore, inhibiting EZH2 suppressed regeneration of hair cells in zebrafish neuromasts and altered expressions of some developmental stage correlated genes.

conclusionsOur results extended the understanding for molecular basis of hair cell regeneration ability and revealed the potential role of Ezh2 in it.

Indexed as

Single-Cell Gene Expression AnalysisAnimalsCell ProliferationEnhancer of Zeste Homolog 2 ProteinHair Cells, AuditoryMiceSignal TransductionEnhancer of Zeste Homolog 2 ProteinEzh2 protein, mouseEZH2Hair cells regenerationSingle-cell transcriptomeSupporting cellsTranscriptional regulator

Identifiers

PMID38281217
OpenAlexW4391290298

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