Proteome Analysis of Serum Purified Using

Daisuke Nakajima, Ryo Konno, Yasuomi Miyashita, Masaki Ishikawa, Osamu Ohara, Yusuke Kawashima

Open access · goldAbstract read

In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.9field-weighted citation impact, top 28% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

Thin-diaPASEF: diaPASEF for maximizing proteome coverage in single-shot proteomics.DNA research : an international journal for rapid publication of reports on genes and genomes · 2025
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Daisuke NakajimaDepartment of Applied Genomics, Kazusa DNA Research Institute, 2-5-23 Kazusa Kamatari, Kisarazu 292-0818, Chiba, Japan.ORCID 0000-0003-3310-3762

Ryo KonnoDepartment of Applied Genomics, Kazusa DNA Research Institute, 2-5-23 Kazusa Kamatari, Kisarazu 292-0818, Chiba, Japan.ORCID 0000-0002-4375-1133

Yasuomi MiyashitaDepartment of Applied Genomics, Kazusa DNA Research Institute, 2-5-23 Kazusa Kamatari, Kisarazu 292-0818, Chiba, Japan.

Masaki IshikawaDepartment of Applied Genomics, Kazusa DNA Research Institute, 2-5-23 Kazusa Kamatari, Kisarazu 292-0818, Chiba, Japan.ORCID 0000-0002-3101-6713

Osamu OharaDepartment of Applied Genomics, Kazusa DNA Research Institute, 2-5-23 Kazusa Kamatari, Kisarazu 292-0818, Chiba, Japan.ORCID 0000-0002-3328-9571

Yusuke KawashimaDepartment of Applied Genomics, Kazusa DNA Research Institute, 2-5-23 Kazusa Kamatari, Kisarazu 292-0818, Chiba, Japan.ORCID 0000-0002-9779-8199

Kazusa DNA Research Institute · JPChiba University · JP

Funding

AMED JP22ek0109586JSPS KAKENHI 23H02465, 22KK0077, 20K20469, and 21K07877

6 · The paper itself

Abstract

Serum and plasma exhibit a broad dynamic range of protein concentrations, posing challenges for proteome analysis. Various technologies have been developed to reduce this complexity, including high-abundance depletion methods utilizing antibody columns, extracellular vesicle enrichment techniques, and trace protein enrichment using nanobead cocktails. Here, we employed lectins to address this, thereby extending the scope of biomarker discovery in serum or plasma using a novel approach. We enriched serum proteins using 37 different lectins and subjected them to LC-MS/MS analysis with data-independent acquisition.

Indexed as

Lupus Erythematosus, SystemicSolanum lycopersicumSolanum tuberosumAnimalsBiomarkersBlood ProteinsChromatography, LiquidLectinsMicePlant LectinsProteomeTandem Mass SpectrometryBiomarkersBlood ProteinsLectinsPlant LectinsProteometomato lectinbiomarkerLycopersicon esculentum lectinplasmaproteomeserumSolanum tuberosum lectin

Identifiers

PMID38279312

DOI10.3390/ijms25021315

PMCPMC10816257

OpenAlexW4391110510

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.