ArticleNature communications2024
Large-scale genomic rearrangements boost SCRaMbLE in Saccharomyces cerevisiae.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 24 citations in OpenAlex.
- Yeast species resource exploration and application in cell factories.Synthetic and systems biotechnology · 2026Review
- Expression of EndonucleaseCurrent issues in molecular biology · 2026Article
- Synthetic rewriting technologies in mammalian cells.Nature communications · 2026Review
- Innovative tools for customized yeast cell factory.Biodesign research · 2025Article
- The design and engineering of synthetic genomes.Nature reviews. Genetics · 2025Review
- Enhancing recombinant protein production through Cre-loxP mediated chromosomal rearrangement evolution in Kluyveromyces marxianus.Communications biology · 2025Article
- Spontaneous and environment induced genomic alterations in yeast model.Cell insight · 2025Review
- Synthetic evolution ofmLife · 2025Review
- Towards the first synthetic eukaryotic cell.Biosafety and health · 2024Review
- Enhancing whole yeast genome rearrangements through multiple LoxPsym sequences.Science China. Life sciences · 2024Article
- Efficient Broad-Spectrum Cyanophage Function Module Mining.Microorganisms · 2024Article
Corrections and comments
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Authors and funding
16 authors at 8 institutions in 3 countries.
Funding
Abstract
Synthetic Chromosome Rearrangement and Modification by LoxP-mediated Evolution (SCRaMbLE) is a promising tool to study genomic rearrangements. However, the potential of SCRaMbLE to study genomic rearrangements is currently hindered, because a strain containing all 16 synthetic chromosomes is not yet available. Here, we construct SparLox83R, a yeast strain containing 83 loxPsym sites distributed across all 16 chromosomes. SCRaMbLE of SparLox83R produces versatile genome-wide genomic rearrangements, including inter-chromosomal events. Moreover, when combined with synthetic chromosomes, SCRaMbLE of hetero-diploids with SparLox83R leads to increased diversity of genomic rearrangements and relatively faster evolution of traits compared to hetero-diploids only with wild-type chromosomes. Analysis of the SCRaMbLEd strain with increased tolerance to nocodazole demonstrates that genomic rearrangements can perturb the transcriptome and 3D genome structure and consequently impact phenotypes. In summary, a genome with sparsely distributed loxPsym sites can serve as a powerful tool for studying the consequence of genomic rearrangements and accelerating strain engineering in Saccharomyces cerevisiae.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.