ArticleDevelopment (Cambridge, England)2024
Spherical harmonics analysis reveals cell shape-fate relationships in zebrafish lateral line neuromasts.
Article in Development (Cambridge, England), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed.
- Single-cell morphodynamics predict cell fate decisions during mucociliary epithelial differentiation.Molecular systems biology · 2026Article
- High-Fidelity Long-term Whole-embryo Lineage and Fate Reconstruction by Iterative Tracking with Error Correction.bioRxiv : the preprint server for biology · 2026Article
- Activity and retinoic acid drive hair cell spatial patterning in the zebrafish utricle.Development (Cambridge, England) · 2026Article
- Single-cell morphodynamics predict cell fate decisions during mucociliary epithelial differentiation.bioRxiv : the preprint server for biology · 2025Article
- Ultrack: pushing the limits of cell tracking across biological scales.Nature methods · 2025Article
- Microtubules regulate tissue-level navigation in skin-resident macrophages.Journal of cell science · 2025Article
- 3D multiscale shape analysis of nuclei and in vivo elastic stress sensors allows force inference.Biophysical journal · 2025Article
- Long-rangeProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Multiple mechanisms of aminoglycoside ototoxicity are distinguished by subcellular localization of action.bioRxiv : the preprint server for biology · 2024Article
- Multiple mechanisms of aminoglycoside ototoxicity are distinguished by subcellular localization of action.Frontiers in neurology · 2024Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors.
Funding
Abstract
Cell shape is a powerful readout of cell state, fate and function. We describe a custom workflow to perform semi-automated, 3D cell and nucleus segmentation, and spherical harmonics and principal components analysis to distill cell and nuclear shape variation into discrete biologically meaningful parameters. We apply these methods to analyze shape in the neuromast cells of the zebrafish lateral line system, finding that shapes vary with cell location and identity. The distinction between hair cells and support cells accounted for much of the variation, which allowed us to train classifiers to predict cell identity from shape features. Using transgenic markers for support cell subpopulations, we found that subtypes had different shapes from each other. To investigate how loss of a neuromast cell type altered cell shape distributions, we examined atoh1a mutants that lack hair cells. We found that mutant neuromasts lacked the cell shape phenotype associated with hair cells, but did not exhibit a mutant-specific cell shape. Our results demonstrate the utility of using 3D cell shape features to characterize, compare and classify cells in a living developing organism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.