ArticleMicroorganisms2023
Direct Impact of the Air on Mutant Cells for Mutagenicity Assessments in Urban Environments.
Article in Microorganisms, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Authors and funding
5 authors.
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Abstract
backgroundUrban air pollution is recognized as a critical problem for public health and is classified as a carcinogen for humans. A great number of studies have focused on the monitoring of urban air mutagenicity. One of the best-known and applied methods for assessing mutagenicity is the Ames test, a bacterial reverse mutation test. The classic protocol for assessing air mutagenicity involves the concentration of particulate matter (PM) on filters and subsequent extraction using organic solvents. This work aimed to develop a method for the evaluation of air mutagenicity directly impacted by air on microbial plates already containing an Ames' microbial sensor.
methodsA specific six-month sampling campaign was carried out in Turin in a period with high air pollution. Samples were tested for mutagenicity on
resultsThe new protocol is able to evaluate the mutagenicity of the sampled air and obtain repeatable results. The final sensitivity is similar to the traditional method (≈10 net revertants/m
conclusionsDespite some critical issues in contamination control, the method is easier, faster, and less expensive than traditional methods.
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