Evidence map›Paper›PMID 38275528›Full record

ArticleBrain sciences2024

Neuropathological Applications of Microscopy with Ultraviolet Surface Excitation (MUSE): A Concordance Study of Human Primary and Metastatic Brain Tumors.

Mirna Lechpammer, Austin Todd, Vivian Tang, Taryn Morningstar, Alexander Borowsky, Kiarash Shahlaie, John A Kintner, John D McPherson, John W Bishop, Farzad Fereidouni and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brain sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Mirna LechpammerDepartment of Pathology and Laboratory Medicine, University of California Davis Health, Sacramento, CA 95817, USA.
Austin ToddDepartment of Pathology and Laboratory Medicine, University of California Davis Health, Sacramento, CA 95817, USA.
Vivian TangDepartment of Pathology and Laboratory Medicine, University of California Davis Health, Sacramento, CA 95817, USA.ORCID 0000-0001-8829-2316
Taryn MorningstarDepartment of Pathology and Laboratory Medicine, University of California Davis Health, Sacramento, CA 95817, USA.
Alexander BorowskyDepartment of Pathology and Laboratory Medicine, University of California Davis Health, Sacramento, CA 95817, USA.
Kiarash ShahlaieDepartment of Neurosurgery, University of California Davis Health, Sacramento, CA 95817, USA.
John A KintnerDepartment of Pathology and Laboratory Medicine, University of California Davis Health, Sacramento, CA 95817, USA.
John D McPhersonDepartment of Biochemistry and Molecular Medicine, University of California Davis Health, Sacramento, CA 95817, USA.
John W BishopDepartment of Pathology and Laboratory Medicine, University of California Davis Health, Sacramento, CA 95817, USA.
Farzad FereidouniDepartment of Pathology and Laboratory Medicine, University of California Davis Health, Sacramento, CA 95817, USA.
Zachary T HarmanyDepartment of Pathology and Laboratory Medicine, University of California Davis Health, Sacramento, CA 95817, USA.ORCID 0000-0002-5871-1783
Nicholas ColeyDepartment of Pathology and Laboratory Medicine, University of California Davis Health, Sacramento, CA 95817, USA.
David ZagzagDepartments of Pathology and Neurosurgery, New York University Langone Medical Center, New York, NY 10016, USA.ORCID 0000-0002-2331-0613
Jason W H WongSchool of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pok Fu Lam, Hong Kong SAR, China.
Jiang TaoKinghorn Centre for Clinical Genomics, Garvan Institute of Medical Research, Darlinghurst NSW 2010, Australia.
Luke B HessonKinghorn Centre for Clinical Genomics, Garvan Institute of Medical Research, Darlinghurst NSW 2010, Australia.
Leslie BurnettKinghorn Centre for Clinical Genomics, Garvan Institute of Medical Research, Darlinghurst NSW 2010, Australia.
Richard LevensonDepartment of Pathology and Laboratory Medicine, University of California Davis Health, Sacramento, CA 95817, USA.

Funding

Staff InvestigatorsP30CA093373 · NCI · UNIVERSITY OF CALIFORNIA DAVIS · PI Laura Marcu · 2002 to 2026
$84.9M
Imaging and informatics techniques to spatially map tumor-associated collagen: novel cancer diagnostic tools (2 of 2)R33CA202881 · NCI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI LEVENSON, RICHARD M. · 2017 to 2019
$1.3M
NCI NIH HHS P30 CA093373NCI NIH HHS R33 CA202881
6 · The paper itself

Abstract

Whereas traditional histology and light microscopy require multiple steps of formalin fixation, paraffin embedding, and sectioning to generate images for pathologic diagnosis, Microscopy using Ultraviolet Surface Excitation (MUSE) operates through UV excitation on the cut surface of tissue, generating images of high resolution without the need to fix or section tissue and allowing for potential use for downstream molecular tests. Here, we present the first study of the use and suitability of MUSE microscopy for neuropathological samples. MUSE images were generated from surgical biopsy samples of primary and metastatic brain tumor biopsy samples (n = 27), and blinded assessments of diagnoses, tumor grades, and cellular features were compared to corresponding hematoxylin and eosin (H&E) images. A set of MUSE-treated samples subsequently underwent exome and targeted sequencing, and quality metrics were compared to those from fresh frozen specimens. Diagnostic accuracy was relatively high, and DNA and RNA integrity appeared to be preserved for this cohort. This suggests that MUSE may be a reliable method of generating high-quality diagnostic-grade histologic images for neuropathology on a rapid and sample-sparing basis and for subsequent molecular analysis of DNA and RNA.

Indexed as

brain neoplasmimagingmicroscopyMUSEneuropathology

Identifiers

PMID38275528
PMCPMC10813539

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.