ArticleScientific data2024
Semi-automated sequence curation for reliable reference datasets in ITS2 vascular plant DNA (meta-)barcoding.
Article in Scientific data, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed.
- Seasonal Diet Variation in Reintroduced Milu (Animals : an open access journal from MDPI · 2026Article
- Real-time PCR to targetFood chemistry. Molecular sciences · 2026Article
- Method comparison of microscopy, metabarcoding, and multispectral imaging flow cytometry for identification and relative abundance analysis of insect-dispersed pollen.Scientific reports · 2026Article
- Advancing plant DNA barcoding: integrating chloroplast genome sequencing, cryptic diversity discovery and machine learning.Molecular biology reports · 2026Review
- Honey bee food resources under threat from climate change.Nature communications · 2025Article
- Questionable Species Names for Distinct Species Clusters: An Empirical Test of the BOLD Molecular Identification Engine.Insects · 2025Article
- Comparing the spring diet of buff-tailed bumble bees and honey bees (Hymenoptera: Apidae) in a forest landscape: a metabarcoding approach.Journal of insect science (Online) · 2025Article
- Endophytic fungi of Cynodon dactylon: a treasure trove of bioactive compounds.Archives of microbiology · 2025Review
- Unveiling Heterospecific Pollen Deposition inEcology and evolution · 2025Article
- Data on the diet and nutrition of urban and rural bumblebees.Scientific data · 2025Article
- COI Metabarcoding as a Novel Approach for Assessing the Honey Bee Source of European Honey.Foods (Basel, Switzerland) · 2025Article
- Integrating DNA Barcoding Within an Orthogonal Approach for Herbal Product Authentication: A Narrative Review.Phytochemical analysis : PCA · 2025Review
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Authors and funding
17 authors.
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Abstract
One of the most critical steps for accurate taxonomic identification in DNA (meta)-barcoding is to have an accurate DNA reference sequence dataset for the marker of choice. Therefore, developing such a dataset has been a long-term ambition, especially in the Viridiplantae kingdom. Typically, reference datasets are constructed with sequences downloaded from general public databases, which can carry taxonomic and other relevant errors. Herein, we constructed a curated (i) global dataset, (ii) European crop dataset, and (iii) 27 datasets for the EU countries for the ITS2 barcoding marker of vascular plants. To that end, we first developed a pipeline script that entails (i) an automated curation stage comprising five filters, (ii) manual taxonomic correction for misclassified taxa, and (iii) manual addition of newly sequenced species. The pipeline allows easy updating of the curated datasets. With this approach, 13% of the sequences, corresponding to 7% of species originally imported from GenBank, were discarded. Further, 259 sequences were manually added to the curated global dataset, which now comprises 307,977 sequences of 111,382 plant species.
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