Evidence map›Paper›PMID 38271396›Full record

ArticlePloS one2024

A polarized cell system amenable to subcellular resolution imaging of influenza virus infection.

Jean-Baptiste Brault, Catherine Thouvenot, Magda Cannata Serio, Sylvain Paisant, Julien Fernandes, David Gény, Lydia Danglot, Adeline Mallet, Nadia Naffakh

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Jean-Baptiste BraultInstitut Pasteur, Université Paris Cité, CNRS UMR 3569, RNA Biology of Influenza Viruses, Paris, France.ORCID 0000-0001-5950-2601
Catherine ThouvenotInstitut Pasteur, Université Paris Cité, C2RT, Ultrastructural BioImaging Unit, Paris, France.
Magda Cannata SerioInstitut Pasteur, Université Paris Cité, CNRS UMR 3569, RNA Biology of Influenza Viruses, Paris, France.ORCID 0000-0002-2084-3406
Sylvain PaisantInstitut Pasteur, Université Paris Cité, CNRS UMR 3569, RNA Biology of Influenza Viruses, Paris, France.
Julien FernandesInstitut Pasteur, Université Paris Cité, C2RT, Unit of Technology and Services Photonic BioImaging, Paris, France.
David GényInstitute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, NeurImag Facility, Université Paris Cité, Paris, France.
Lydia DanglotInstitute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, NeurImag Facility, Université Paris Cité, Paris, France.
Adeline MalletInstitut Pasteur, Université Paris Cité, C2RT, Ultrastructural BioImaging Unit, Paris, France.
Nadia NaffakhInstitut Pasteur, Université Paris Cité, CNRS UMR 3569, RNA Biology of Influenza Viruses, Paris, France.ORCID 0000-0002-0424-0277
Centre National de la Recherche Scientifique · FRInstitut Pasteur · FRInserm · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The life cycle of influenza A viruses (IAV), and notably intracellular trafficking of the viral genome, depends on multiple interactions with the cellular cytoskeleton and endomembrane system. A limitation of the conventional cellular models used for mechanistic study and subcellular imaging of IAV infection is that they are cultured in two dimensions (2D) under non-polarizing conditions, and therefore they do not recapitulate the intracellular organization of the polarized respiratory epithelial cells naturally targeted by IAVs. To overcome this limitation, we developed an IAV-infection assay in a 3D cell culture system which allows imaging along the baso-lateral axis of polarized cells, with subcellular resolution. Here we describe a protocol to grow polarized monolayers of Caco2-TC7 cells on static Cytodex-3 microcarrier beads, infect them with IAV, and subsequently perform immunostaining and confocal imaging, or electron microscopy, on polarized IAV-infected cells. This method can be extended to other pathogens that infect human polarized epithelial cells.

Indexed as

Influenza A virusInfluenza, HumanOrthomyxoviridae InfectionsCaco-2 CellsEpithelial CellsHumansVirus Replication

Identifiers

PMID38271396
PMCPMC10810476
OpenAlexW4391216090

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.