Evidence map›Paper›PMID 38268973›Full record

ArticleBio-protocol2024

The Development of an Advanced Model for Multilayer Human Skin Reconstruction In Vivo.

Maryna Pavlova, Velmurugan Balaiya, Jocelyn C Flores, Michael Ferreyros, Katie Bush, Amy Hopkin, Igor Kogut, Dennis R Roop, Ganna Bilousova

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 59% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Maryna PavlovaDepartment of Dermatology, Gates Institute, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Velmurugan BalaiyaDepartment of Dermatology, Gates Institute, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Jocelyn C FloresDepartment of Dermatology, Gates Institute, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Michael FerreyrosDepartment of Dermatology, Gates Institute, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Katie BushAVITA Medical, Valencia, CA, USA.
Amy HopkinAVITA Medical, Valencia, CA, USA.
Igor KogutDepartment of Dermatology, Gates Institute, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Dennis R RoopDepartment of Dermatology, Gates Institute, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Ganna BilousovaDepartment of Dermatology, Gates Institute, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
University of Colorado Anschutz Medical Campus · US

Funding

Developing a platform for human somatic cell rejuvenation, expansion and genetic engineering using synthetic RNA moleculesR01AR078551 · NIAMS · UNIVERSITY OF COLORADO DENVER · PI KOGUT, IGOR · 2021 to 2025
$2.3M
Testing the Therapeutic Potential of iPS Cells for Inherited Skin DiseasesR01AR059947 · NIAMS · UNIVERSITY OF COLORADO DENVER · PI ROOP, DENNIS · 2012 to 2017
$2.1M
Exploring Alternative iPS Cell Therapies for Recessive Dystrophic Epidermolysis BullosaU01AR075932 · NIAMS · UNIVERSITY OF COLORADO DENVER · PI BILOUSOVA, GANNA, ROOP, DENNIS · 2019 to 2020
$808k
NIAMS NIH HHS R01 AR059947NIAMS NIH HHS R01 AR078551NIAMS NIH HHS U01 AR075932
6 · The paper itself

Abstract

Human skin reconstruction on immune-deficient mice has become indispensable for in vivo studies performed in basic research and translational laboratories. Further advancements in making sustainable, prolonged skin equivalents to study new therapeutic interventions rely on reproducible models utilizing patient-derived cells and natural three-dimensional culture conditions mimicking the structure of living skin. Here, we present a novel step-by-step protocol for grafting human skin cells onto immunocompromised mice that requires low starting cell numbers, which is essential when primary patient cells are limited for modeling skin conditions. The core elements of our method are the sequential transplantation of fibroblasts followed by keratinocytes seeded into a fibrin-based hydrogel in a silicone chamber. We optimized the fibrin gel formulation, timing for gel polymerization in vivo, cell culture conditions, and seeding density to make a robust and efficient grafting protocol. Using this approach, we can successfully engraft as few as 1.0 × 10

Indexed as

Fibrin-based hydrogelHuman skin equivalentIn vivo skin modelMultilayered skin graftRegenerative medicine

Identifiers

PMID38268973
PMCPMC10804244
OpenAlexW4390506117

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.