Evidence map›Paper›PMID 38265720›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Isolation of Rhizobial Extracellular Membrane Vesicles from Bacteroids.

Paula Ayala-García, Irene Jiménez-Guerrero, Mathias Müsken, Francisco Javier Ollero, José Manuel Borrero-De Acuña, Francisco Pérez-Montaño

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Paula Ayala-GarcíaDepartment of Microbiology, University of Seville, Seville, Spain.
Irene Jiménez-GuerreroDepartment of Microbiology, University of Seville, Seville, Spain.
Mathias MüskenCentral Facility for Microscopy, Helmholtz Centre for Infection Research, Braunschweig, Germany.
Francisco Javier OlleroDepartment of Microbiology, University of Seville, Seville, Spain.
José Manuel Borrero-De AcuñaDepartment of Microbiology, University of Seville, Seville, Spain.
Francisco Pérez-MontañoDepartment of Microbiology, University of Seville, Seville, Spain. fperezm@us.es.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Extracellular-membrane vesicles (EMVs) are spherical buds of the extracellular membrane, commonly produced by Gram-negative bacteria, known to mediate intricate inter-kingdom communication. In this context, comprehensive research dissecting the role of EMVs in one of the most complex nature-occurring molecular dialogues, rhizobium-legume symbiosis, has been so far neglected. During the different stages of the symbiotic process, rhizobia and their host plants establish a very specific and controlled intercellular trafficking of signal molecules. Thus, as conveyors of a broad range of molecules into the target cell, EMVs are gaining weight in the field. Here, we describe a detailed protocol to isolate EMVs from bacteroids of legume nodules, opening a new door for discovering new authors of the symbiotic process.

Indexed as

Extracellular VesiclesFabaceaeRhizobiumMembranesSymbiosisVegetablesBacteroidsExtracellular membrane vesiclesLegumerhizobiumSymbiosisSymbiosome

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.