ArticleMethods in molecular biology (Clifton, N.J.)2024
Construction of a Mycoviral Infectious Clone for Reverse Genetics in Botrytis cinerea.
Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- Botrytis cinerea Protoplasts Production and Inoculation.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Identification of Mycoviral Infections.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Mycologists and Virologists Align: ProposingViruses · 2024Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The most important advances in our understanding of the viral life cycle, such as genome replication, packaging, transmission, and host interactions, have been made via the development of viral infectious full-length clones. Here, we describe the detailed protocols for the construction of an infectious clone derived from Botrytis virus F (BVF), a mycoflexivirus infecting the plant pathogenic fungus Botrytis cinerea, the determination of the complete sequence of the cloned mycovirus, the preparation of fungal protoplasts, and the transfection of protoplasts using transcripts derived from the BVF infectious clone.
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Registered trials
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