ArticleApplied and environmental microbiology2024
Combined pH ratiometry and fluorescence lectin-binding analysis (pH-FLBA) for microscopy-based analyses of biofilm pH and matrix carbohydrates.
Article in Applied and environmental microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 6 citations in OpenAlex.
- Arginine modulates the pH, microbial composition, and matrix architecture of biofilms from caries-active patients.International journal of oral science · 2025Trial
- pH-OBiofilm · 2026Article
- The effect of probiotic bacteria on community composition, microscale pH and matrix architecture in a saliva-derived model of oral biofilm.Journal of oral microbiology · 2026Article
- The effect of multiple-enzyme treatment onBiofilm · 2025Article
- pH-FISH: coupled microscale analysis of microbial identity and acid-base metabolism in complex biofilm samples.Microbiome · 2024Article
- Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
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Abstract
Bacterial biofilms have a complex and heterogeneous three-dimensional architecture that is characterized by chemically and structurally distinct microenvironments. Confocal microscopy-based pH ratiometry and fluorescence lectin-binding analysis (FLBA) are well-established methods to characterize pH developments and the carbohydrate matrix architecture of biofilms at the microscale. Here, we developed a combined analysis, pH-FLBA, to concomitantly map biofilm pH and the distribution of matrix carbohydrates in bacterial biofilms while preserving the biofilm microarchitecture. As a proof of principle, the relationship between pH and the presence of galactose- and fucose-containing matrix components was investigated in dental biofilms grown with and without sucrose. The pH response to a sucrose challenge was monitored in different areas at the biofilm base using the ratiometric pH-sensitive dye C-SNARF-4. Thereafter, the fucose- and galactose-specific fluorescently labeled lectins
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