Evidence map›Paper›PMID 38263491›Full record

ArticleGlycobiology2024

ppmFixer: a mass error adjustment for pGlyco3.0 to correct near-isobaric mismatches.

Trevor M Adams, Peng Zhao, Rui Kong, Lance Wells

Abstract read
In one paragraph

Article in Glycobiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Trevor M AdamsDepartment of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens 30602, Georgia.
Peng ZhaoDepartment of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens 30602, Georgia.ORCID 0000-0002-9296-5293
Rui KongDepartment of Pathology and Laboratory Medicine, Emory Vaccine Center, Emory University, 7 Frist Ave, Atlanta 30317, Georgia.
Lance WellsDepartment of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens 30602, Georgia.ORCID 0000-0003-4956-5363

Funding

HIV-1 Fusion Peptide-directed Vaccine Design Using Virus-like ParticlesR01AI162267 · NIAID · EMORY UNIVERSITY · PI Rui Kong · 2021 to 2026
$5.7M
Structure and Function in alpha-Dystroglycan GlycosylationR01GM111939 · NIGMS · UNIVERSITY OF GEORGIA · PI Lance Wells · 2014 to 2026
$4.0M
Origin of N-Glycan Site-Specific HeterogeneityR01GM130915 · NIGMS · UNIVERSITY OF GEORGIA · PI KANNAN, NATARAJAN, MOREMEN, KELLEY W. · 2019 to 2022
$3.4M
Glycoscience Training ProgramT32GM107004 · NIGMS · UNIVERSITY OF GEORGIA · PI AMSTER, I JONATHAN, HALTIWANGER, ROBERT S. · 2014 to 2021
$1.3M
NIAID NIH HHS R01 AI162267NIGMS NIH HHS R01 GM111939NIGMS NIH HHS R01 GM130915NIGMS NIH HHS T32 GM107004NIH HHS R01AI162267
6 · The paper itself

Abstract

Modern glycoproteomics experiments require the use of search engines due to the generation of countless spectra. While these tools are valuable, manual validation of search engine results is often required for detailed analysis of glycopeptides as false-discovery rates are often not reliable for glycopeptide data. Near-isobaric mismatches are a common source of misidentifications for the popular glycopeptide-focused search engine pGlyco3.0, and in this technical note we share a strategy and script that improves the accuracy of the search utilizing two manually validated datasets of the glycoproteins CD16a and HIV-1 Env as proof-of-principle.

Indexed as

ProteomicsTandem Mass SpectrometryGlycopeptidesGlycosylationSearch EngineGlycopeptidesGlycopeptideglycoproteinGlycoproteomicsmass spectrometry

Identifiers

PMID38263491
PMCPMC11005163

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.