Evidence map›Paper›PMID 38263329›Full record

ArticleEMBO reports2024

ChemRAP uncovers specific mRNA translation regulation via RNA 5' phospho-methylation.

Hélène Ipas, Ellen B Gouws, Nathan S Abell, Po-Chin Chiou, Sravan K Devanathan, Solène Hervé, Sidae Lee, Marvin Mercado, Calder Reinsborough, Levon Halabelian and 2 more

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in EMBO reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 99% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 2 countries.

Hélène Ipas *Department of Molecular Biosciences, University of Texas at Austin, 2500 Speedway, 78712, Austin, TX, USA.
Ellen B Gouws *Department of Molecular Biosciences, University of Texas at Austin, 2500 Speedway, 78712, Austin, TX, USA.
Nathan S Abell *Department of Molecular Biosciences, University of Texas at Austin, 2500 Speedway, 78712, Austin, TX, USA.ORCID 0000-0003-1098-5395
Po-Chin Chiou *Department of Molecular Biosciences, University of Texas at Austin, 2500 Speedway, 78712, Austin, TX, USA.ORCID 0009-0008-0780-1966
Sravan K Devanathan *Department of Molecular Biosciences, University of Texas at Austin, 2500 Speedway, 78712, Austin, TX, USA.
Solène Hervé *Department of Molecular Biosciences, University of Texas at Austin, 2500 Speedway, 78712, Austin, TX, USA.ORCID 0000-0002-5015-8493
Sidae Lee *Department of Molecular Biosciences, University of Texas at Austin, 2500 Speedway, 78712, Austin, TX, USA.
Marvin Mercado *Department of Molecular Biosciences, University of Texas at Austin, 2500 Speedway, 78712, Austin, TX, USA.
Calder Reinsborough *Department of Molecular Biosciences, University of Texas at Austin, 2500 Speedway, 78712, Austin, TX, USA.
Levon HalabelianStructural Genomics Consortium, and Princess Margaret Cancer Centre, University of Toronto, Toronto, ON, M5G 2M9, Canada.
Cheryl H ArrowsmithStructural Genomics Consortium, and Princess Margaret Cancer Centre, University of Toronto, Toronto, ON, M5G 2M9, Canada.
Blerta XhemalçeDepartment of Molecular Biosciences, University of Texas at Austin, 2500 Speedway, 78712, Austin, TX, USA. b.xhemalce@austin.utexas.edu.ORCID 0000-0002-0517-9607
The University of Texas at Austin · USUniversity of Toronto · CA

Funding

Elucidating mechanisms of microRNA pathway deregulation in human cellsR01GM127802 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI XHEMALCE, BLERTA · 2018 to 2022
$1.7M
NIGMS NIH HHS R01 GM127802Wellcome Trust
6 · The paper itself

Abstract

5'-end modifications play key roles in determining RNA fates. Phospho-methylation is a noncanonical cap occurring on either 5'-PPP or 5'-P ends. We used ChemRAP, in which affinity purification of cellular proteins with chemically synthesized modified RNAs is coupled to quantitative proteomics, to identify 5'-Pme "readers". We show that 5'-Pme is directly recognized by EPRS, the central subunit of the multisynthetase complex (MSC), through its linker domain, which has previously been involved in key noncanonical EPRS and MSC functions. We further determine that the 5'-Pme writer BCDIN3D regulates the binding of EPRS to specific mRNAs, either at coding regions rich in MSC codons, or around start codons. In the case of LRPPRC (leucine-rich pentatricopeptide repeat containing), a nuclear-encoded mitochondrial protein associated with the French Canadian Leigh syndrome, BCDIN3D deficiency abolishes binding of EPRS around its mRNA start codon, increases its translation but ultimately results in LRPPRC mislocalization. Overall, our results suggest that BCDIN3D may regulate the translation of specific mRNA via RNA-5'-Pme.

Indexed as

Neoplasm ProteinsProtein BiosynthesisCanadaMethylationRNARNA, MessengerNeoplasm ProteinsRNARNA, MessengerBCDIN3DLocal TranslationLRPPRCRNA Modification ReaderRNA Phospho-methylation

Identifiers

PMID38263329
PMCPMC10933402
OpenAlexW4391131308

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.