ArticleCell communication and signaling : CCS2024
Immunophenotype profile by flow cytometry reveals different subtypes of extracellular vesicles in porcine seminal plasma.
Article in Cell communication and signaling : CCS, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- Seminal Extracellular Vesicles as Molecular Modulators of Male Reproductive Function.Reproduction in domestic animals = Zuchthygiene · 2026Review
- Optimizing plasma-derived small extracellular vesicle purification to enhance downstream applications.iScience · 2026Article
- Seminal Plasma Exosomes in Male Infertility: Roles, Mechanisms, and Applications-A Double-Edged Sword.The world journal of men's health · 2026Review
- Bovine oviductal and uterine extracellular vesicles enhance blastocyst development in confined individual embryo culture.Journal of assisted reproduction and genetics · 2026Article
- Changes in the microRNA cargo of granulosa cell-derived extracellular vesicles under oxidative stress in a bovine model.Biological research · 2026Article
- Bronchial epithelial cell-derived extracellular vesicle analysis using conventional, imaging, and nanoscale flow cytometry technologies.Scientific reports · 2026Article
- Proteomic profiling of porcine seminal extracellular vesicles reveals potential in vivo fertility biomarkers.Andrology · 2026Article
- Review
- Enhancing developmental potential of vitrified in vitro matured bovine oocytes using extracellular vesicles from large follicles.Scientific reports · 2025Article
- Extracellular Vesicles in Reproduction: Biology, Production, and Potential Applications in Livestock Breeding.Reproduction in domestic animals = Zuchthygiene · 2025Review
- Male reproductive tract extracellular vesicles display region-specific heterogeneity in mice.Reproduction (Cambridge, England) · 2025Article
- Protective role of extracellular vesicles against oxidative DNA damage.Biological research · 2025Article
- Flow virometry: recent advancements, best practices, and future frontiers.Journal of virology · 2025Review
- Exosomal ncRNAs in reproductive cancers†.Biology of reproduction · 2025Review
- Multi-Omics Sequencing Dissects the Atlas of Seminal Plasma Exosomes from Semen Containing Low or High Rates of Sperm with Cytoplasmic Droplets.International journal of molecular sciences · 2025Article
- Relationship of early pregnancy maternal plasma progesterone concentrations on piglet birth weight, within-litter birth weight variation, and piglet mortality from crossbred Landrace or Yorkshire dams bred with purebred Landrace or Yorkshire sires.Journal of animal science · 2025Article
- RNA profiles differ between small and large extracellular vesicle subsets isolated from porcine seminal plasma.BMC genomics · 2024Article
- Updating Research on Extracellular Vesicles of the Male Reproductive Tract in Farm Animals: A Systematic Review.Animals : an open access journal from MDPI · 2024Review
- Cryogenic electron microscopy reveals morphologically distinct subtypes of extracellular vesicles among porcine ejaculate fractions.Scientific reports · 2024Article
- Small and Large Extracellular Vesicles of Porcine Seminal Plasma Differ in Lipid Profile.International journal of molecular sciences · 2024Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPorcine seminal plasma (SP) is endowed with a heterogeneous population of extracellular vesicles (sEVs). This study evaluated the immunophenotypic profile by high-sensitivity flow cytometry of eight sEV subpopulations isolated according to their size (small [S-sEVs] and large [L-sEVs]) from four different SP sources, namely three ejaculate fractions (the first 10 mL of the sperm rich fraction [SRF-P1], the remaining SRF [SRF-P2], and the post-SRF [PSRF]) and entire ejaculate (EE).
methodsSeminal EVs were isolated using a size exclusion chromatography-based protocol from six SP pools (five ejaculates/pool) of each SP source and characterized using complementary approaches including total protein (BCA™assay), particle size distribution (dynamic light scattering), morphology (transmission electron microscopy), and purity (albumin by Western blot). Expression of CD9, CD63, CD81, CD44 and HSP90β was analyzed in all sEV subpopulations by high-sensitivity flow cytometry according to MIFlowCyt-EV guidelines, including an accurate calibration, controls, and discrimination by CFSE-labelling.
resultsEach sEV subpopulation exhibited a specific immunophenotypic profile. The percentage of sEVs positive for CD9, CD63, CD81 and HSP90β differed between S- and L-sEVs (P < 0.0001). Specifically, the percentage of sEVs positive for CD9 and CD63 was higher and that for CD81 was lower in S- than L-sEVs in the four SP sources. However, the percentage of HSP90β-positive sEVs was lower in S-sEVs than L-sEVs in the SRF-P1 and EE samples. The percentage of sEVs positive for CD9, CD63, and CD44 also differed among the four SP sources (P < 0.0001), being highest in PSRF samples. Notably, virtually all sEV subpopulations expressed CD44 (range: 88.04-98.50%).
conclusionsThis study demonstrated the utility of high-sensitivity flow cytometry for sEV immunophenotyping, allowing the identification of distinct sEV subpopulations that may have different cellular origin, cargo, functions, and target cells.
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