Evidence map›Paper›PMID 38261068›Full record

ArticleMicrobial ecology2024

Diversity and ecological function of urease-producing bacteria in the cultivation environment of Gracilariopsis lemaneiformis.

Pengbing Pei, Muhammad Aslam, Hui Wang, Peilin Ye, Tangcheng Li, Honghao Liang, Qi Lin, Weizhou Chen, Hong Du

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial ecology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
7.2field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
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  5. Article
  6. RhizosphericFrontiers in microbiology · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Pengbing PeiGuangdong Provincial Key Laboratory of Marine Biotechnology, College of Science, Shantou University, Shantou, 515063, China.
Muhammad AslamGuangdong Provincial Key Laboratory of Marine Biotechnology, College of Science, Shantou University, Shantou, 515063, China.
Hui WangGuangdong Provincial Key Laboratory of Marine Biotechnology, College of Science, Shantou University, Shantou, 515063, China.
Peilin YeGuangdong Provincial Key Laboratory of Marine Biotechnology, College of Science, Shantou University, Shantou, 515063, China.
Tangcheng LiGuangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, College of Science, Shantou University, Shantou, 515063, China.
Honghao LiangGuangdong Provincial Key Laboratory of Marine Biotechnology, College of Science, Shantou University, Shantou, 515063, China.
Qi LinKey Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen, 361000, China.
Weizhou ChenGuangdong Provincial Key Laboratory of Marine Biotechnology, College of Science, Shantou University, Shantou, 515063, China.
Hong DuGuangdong Provincial Key Laboratory of Marine Biotechnology, College of Science, Shantou University, Shantou, 515063, China. hdu@stu.edu.cn.
Shantou University · CNFujian Fisheries Research Institute · CN

Funding

the National Natural Science Foundation of China 41976125the Natural Science Foundation of China grants 42206116the Open Program of Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province 2022fjscq02the Program for University Innovation Team of Guangdong Province 2022KCXTD008the Team Project of Department of Education of Guangdong Province 2018KCXTD012
6 · The paper itself

Abstract

Urease-producing bacteria (UPB) provide inorganic nitrogen for primary producers by hydrolyzing urea, and play an important role in marine nitrogen cycle. However, there is still an incomplete understanding of UPB and their ecological functions in the cultivation environment of the red macroalgae Gracilariopsis lemaneiformis. This study comprehensively analyzed the diversity of culturable UPB and explored their effects on urea uptake by G. lemaneiformis. A total of 34 isolates belonging to four main bacterial phyla i.e. (Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria) were identified through 16S rRNA sequencing and were screened for UPB by urea agar chromogenic medium assay and ureC gene cloning. Our data revealed that only 8 strains contained urease. All of these UPB exhibited different urease activities, which were determined by the Berthelot reaction colorimetry assay. Additionally, the UPB strain (G13) isolated from G. lemaneiformis with higher urease activity was selected for co-culture with G. lemaneiformis to explore its role in promoting or inhibiting nitrogen uptake by macroalgae. The results showed a significant increase in urea consumption in the culture medium and the total cellular nitrogen in G. lemaneiformis in the UPB-co culture group compared to the sterile group. This suggests that the selected UPB strain positively influences nitrogen uptake by G. lemaneiformis. Similarly, isotopic assays revealed that the δ

Indexed as

SeaweedUreaseBacteriaNitrogenRNA, Ribosomal, 16SUreaNitrogenRNA, Ribosomal, 16SUreaUreaseDiversityEcological functionGracilariopsis lemaneiformisUreaseUrease-producing bacteria (UPB)

Identifiers

PMID38261068
PMCPMC10806000
OpenAlexW4391138075

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.