Evidence map›Paper›PMID 38260617›Full record

ArticlebioRxiv : the preprint server for biology2025

Spatial characterization of interface dermatitis in cutaneous lupus reveals novel chemokine ligand-receptor pairs that drive disease.

Saeed Shakiba, Nazgol-Sadat Haddadi, Khashayar Afshari, Janet E Lubov, Haya S Raef, Robert Li, Ümmügülsüm Yildiz-Altay, Mridushi Daga, Maggi Ahmed Refat, Evangeline Kim and 19 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

29 authors at 3 institutions in 1 country.

Saeed ShakibaUMass Chan Medical School, Dept of Dermatology, Worcester, MA, USA.
Nazgol-Sadat HaddadiUMass Chan Medical School, Dept of Dermatology, Worcester, MA, USA.
Khashayar AfshariUMass Chan Medical School, Dept of Dermatology, Worcester, MA, USA.
Janet E LubovUMass Chan Medical School, Dept of Dermatology, Worcester, MA, USA.
Haya S RaefUMass Chan Medical School, Dept of Dermatology, Worcester, MA, USA.
Robert LiUMass Chan Medical School, Dept of Dermatology, Worcester, MA, USA.
Ümmügülsüm Yildiz-AltayUMass Chan Medical School, Dept of Dermatology, Worcester, MA, USA.
Mridushi DagaUMass Chan Medical School, Dept of Dermatology, Worcester, MA, USA.
Maggi Ahmed RefatUMass Chan Medical School, Dept of Dermatology, Worcester, MA, USA.
Evangeline KimUMass Chan Medical School, Dept of Dermatology, Worcester, MA, USA.
Johanna Galindo de LaflinUMass Chan Medical School, Dept of Dermatology, Worcester, MA, USA.
Andressa AkabaneUMass Chan Medical School, Dept of Dermatology, Worcester, MA, USA.
Priscilla RomanoUMass Chan Medical School, Dept of Dermatology, Worcester, MA, USA.
Jane VongvirathUMass Chan Medical School, Dept of Dermatology, Worcester, MA, USA.
Shany ShermanUMass Chan Medical School, Dept of Dermatology, Worcester, MA, USA.
Elizabeth MacDonaldUMass Chan Medical School, Dept of Dermatology, Worcester, MA, USA.
James P StrassnerUMass Chan Medical School, Dept of Dermatology, Worcester, MA, USA.
Liang ZhangNanoString Technologies, Seattle, WA, USA.
Michael LeonNanoString Technologies, Seattle, WA, USA.
Christina E BaerUMass Chan Medical School, Sanderson Center for Optical Experimentation, Dept of Microbiology and Physiological Systems, Worcester, MA, USA.
Karen DresserUMass Chan Medical School, Dept of Pathology, Worcester, MA, USA.
Yan LiangNanoString Technologies, Seattle, WA, USA.
James B WhitleyDartmouth Hitchcock Medical Center, Dept of Medicine, Lebanon, NH, USA.
Sladjana Skopelja-GardnerDartmouth Hitchcock Medical Center, Dept of Medicine, Lebanon, NH, USA.
John E HarrisUMass Chan Medical School, Dept of Dermatology, Worcester, MA, USA.
April DengUMass Chan Medical School, Dept of Pathology, Worcester, MA, USA.
Matthew D VeselyYale University School of Medicine, Dept of Dermatology, New Haven, CT, USA.
Mehdi RashighiUMass Chan Medical School, Dept of Dermatology, Worcester, MA, USA.
Jillian RichmondUMass Chan Medical School, Dept of Dermatology, Worcester, MA, USA.ORCID 0000-0003-1589-6770
UMass Memorial Health Care · USDartmouth–Hitchcock Medical Center · USUniversity of Massachusetts Chan Medical School · US

Funding

University of Massachusetts Center for Clinical and Translational ScienceUL1TR000161 · NCATS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI LUZURIAGA, KATHERINE F · 2012 to 2015
$11.0M
Innate Immunity Training ProgramT32AI095213 · NIAID · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI SILVERMAN, NEAL · 2011 to 2025
$2.5M
Treg Migration and Function During Autoimmunity within Peripheral TissueR01AR069114 · NIAMS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI HARRIS, JOHN E · 2015 to 2020
$2.2M
Role of VISTA in discoid lupus erythematosusK08AR080777 · NIAMS · YALE UNIVERSITY · PI Matthew D Vesely · 2022 to 2026
$858k
Targeting IFN gamma and chemokines to treat vitiligo in a humanized mouse modelK08AR061437 · NIAMS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI HARRIS, JOHN E · 2011 to 2015
$619k
VISTA regulates type I interferon response to ultraviolet lightR21AR079661 · NIAMS · DARTMOUTH-HITCHCOCK CLINIC · PI SKOPELJA-GARDNER, SLADJANA · 2021 to 2021
$384k
MOLECULAR MECHANISM OF MYOSIN IX MOTILITYF32AR008641 · NIAMS · UNIVERSITY OF PENNSYLVANIA · PI YENGO, CHRISTOPHER M · 2001 to 2003
$116k
NCATS NIH HHS UL1 TR000161NIAID NIH HHS T32 AI095213NIAMS NIH HHS F32 AR008641NIAMS NIH HHS K08 AR061437NIAMS NIH HHS K08 AR080777NIAMS NIH HHS R01 AR069114NIAMS NIH HHS R21 AR079661
6 · The paper itself

Abstract

Background: Chemokines play critical roles in the recruitment and activation of immune cells in both homeostatic and pathologic conditions. Here, we examined chemokine ligand-receptor pairs to better understand the immunopathogenesis of cutaneous lupus erythematosus (CLE), a complex autoimmune connective tissue disorder. Objectives: Our objectives were to (1) characterize the cellular and proteomic constitution of interface dermatitis in CLE using blister biopsies, (2) map chemokine:ligand receptor pairs that govern recruitment of immune cells to form interface dermatitis in CLE, and (3) perform unbiased analyses in tandem on different clinical subtypes to identify novel genes and proteins underlying discoid versus subacute CLE. Methods: We used suction blister biopsies to measure cellular infiltrates with spectral flow cytometry in the interface dermatitis reaction, as well as 184 protein analytes in interstitial skin fluid using 96-plex immunoassay targeted proteomics. Flow and 96-plex immunoassay data concordantly demonstrated significant increases in T cells and antigen presenting cells (APCs). We also performed spatial transcriptomics and spatial proteomics of punch biopsies using digital spatial profiling (DSP) technology on CLE skin and healthy margin controls to examine discreet locations within the tissue. Results: Spatial and 96-plex immunoassay data confirmed elevation of interferon (IFN) and IFN-inducible CXCR3 chemokine ligands. Comparing involved versus uninvolved keratinocytes in CLE samples revealed upregulation of essential inflammatory response genes in areas near interface dermatitis, including Conclusions: Taken together, our data map a pathway from keratinocyte injury to lymphocyte recruitment in CLE via AIM2-Casp8-IL-18-CXCL6/CXCR1 and CCL8/CCR2, and IFNG/IFNL1-CXCL9/CXCL11-CXCR3, and identify potential novel biomarkers of disease.

Identifiers

PMID38260617
PMCPMC10802382
OpenAlexW4390658793

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.