Evidence map›Paper›PMID 38260610›Full record

ArticlebioRxiv : the preprint server for biology2024

Standardized Parts for Activation of Small GTPase Signaling in Living Cells.

Yuchen He, Benjamin M Faulkner, Meaghan A Roberti, Dana K Bassford, Cliff I Stains

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Yuchen HeDepartment of Chemistry, University of Virginia, Charlottesville, VA 22904, USA.
Benjamin M FaulknerDepartment of Chemistry, University of Virginia, Charlottesville, VA 22904, USA.
Meaghan A RobertiDepartment of Chemistry, University of Virginia, Charlottesville, VA 22904, USA.
Dana K BassfordDepartment of Chemistry, University of Virginia, Charlottesville, VA 22904, USA.
Cliff I StainsDepartment of Chemistry, University of Virginia, Charlottesville, VA 22904, USA.ORCID 0000-0002-3165-4539
University of Virginia · USUniversity of Virginia Cancer Center

Funding

Chemical Approaches for Interrogating Fundamental Biomedical ProcessesR35GM148221 · NIGMS · UNIVERSITY OF VIRGINIA · PI Cliff I Stains · 2023 to 2026
$1.6M
Leica tauSTED super resolution microscopy for molecular imaging in fixed and live specimensS10OD030409 · OD · UNIVERSITY OF VIRGINIA · PI PERIASAMY, AMMASI · 2022 to 2022
$850k
NIGMS NIH HHS R35 GM148221NIH HHS S10 OD030409
6 · The paper itself

Abstract

Small GTPases comprise a superfamily of over 167 proteins in the human genome and are critical regulators of a variety of pathways including cell migration and proliferation. Despite the importance of these proteins in cell signaling, a standardized approach for controlling small GTPase activation within living cells is lacking. Herein, we report a split-protein-based approach to directly activate small GTPase signaling in living cells. Importantly, our fragmentation site can be applied across the small GTPase superfamily. We highlight the utility of these standardized parts by demonstrating the ability to directly modulate the activity of four different small GTPases with user-defined inputs, providing a plug and play system for direct activation of small GTPases in living cells.

Identifiers

PMID38260610
PMCPMC10802329
OpenAlexW4390656926

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.