Evidence map›Paper›PMID 38256226›Full record

ArticleInternational journal of molecular sciences2024

Establishment of Polydopamine-Modified HK-2 Cell Membrane Chromatography and Screening of Active Components from

Hongxue Gao, Zhiqiang Liu, Fengrui Song, Junpeng Xing, Zhong Zheng, Zong Hou, Shu Liu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Hongxue GaoState Key Laboratory of Electroanalytical Chemistry & Jilin Provincial Key Laboratory of Chinese Medicine Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Zhiqiang LiuState Key Laboratory of Electroanalytical Chemistry & Jilin Provincial Key Laboratory of Chinese Medicine Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Fengrui SongState Key Laboratory of Electroanalytical Chemistry & Jilin Provincial Key Laboratory of Chinese Medicine Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Junpeng XingState Key Laboratory of Electroanalytical Chemistry & Jilin Provincial Key Laboratory of Chinese Medicine Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Zhong ZhengState Key Laboratory of Electroanalytical Chemistry & Jilin Provincial Key Laboratory of Chinese Medicine Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Zong HouState Key Laboratory of Electroanalytical Chemistry & Jilin Provincial Key Laboratory of Chinese Medicine Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Shu LiuState Key Laboratory of Electroanalytical Chemistry & Jilin Provincial Key Laboratory of Chinese Medicine Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Chinese Academy of Sciences · CNChangchun Institute of Applied Chemistry · CN

Funding

National Natural Science Foundation of China 81872969The Science and Technology Innovation Cooperation Project of Changchun Science and Technology Bureau and Chinese Academy of Sciences No. 22SH14the Science and the Youth Innovation Promotion Association of CAS 2019227
6 · The paper itself

Abstract

Cell membrane chromatography (CMC) has been widely recognized as a highly efficient technique for in vitro screening of active compounds. Nevertheless, conventional CMC approaches suffer from a restricted repertoire of cell membrane proteins, making them susceptible to oversaturation. Moreover, the binding mechanism between silica gel and proteins primarily relies on intermolecular hydrogen bonding, which is inherently unstable and somewhat hampers the advancement of CMC. Consequently, this investigation aimed to establish a novel CMC column that could augment protein loading, enhance detection throughput, and bolster binding affinity through the introduction of covalent bonding with proteins. This study utilizes polydopamine (PDA)-coated silica gel, which is formed through the self-polymerization of dopamine (DA), as the carrier for the CMC column filler. The objective is to construct the HK-2/SiO

Indexed as

GoutIndolesPlantagoPolymersCell MembraneChromatographyExcipientsHumansKidneyMembrane ProteinsSilica GelSilicon DioxideExcipientsIndolesMembrane ProteinspolydopaminePolymersSilica GelSilicon Dioxidecell membrane chromatographyPlantago asiatica L.polydopaminesilica geluric acid

Identifiers

PMID38256226
PMCPMC10816010
OpenAlexW4390977469

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.