Evidence map›Paper›PMID 38255666›Full record

ArticleLife (Basel, Switzerland)2023

Evaluation of the Precision of Kinetic Stem Cell (KSC) Counting for Specific Quantification of Human Mesenchymal Stem Cells in Heterogeneous Tissue Cell Preparations.

Hitesh Chopra, Michael P Daley, Adhya Kumar, James Sugai, Alex Dahlkemper, Darnell Kaigler, James L Sherley

Open access · goldAbstract read
In one paragraph

Article in Life (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Hitesh ChopraDepartment of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI 48109, USA.ORCID 0000-0002-4125-9946
Michael P DaleyInstitute for Applied Life Sciences, University of Massachusetts, Amherst, MA 01003, USA.
Adhya KumarDrik LLC, Oklahoma City, OK 73104, USA.
James SugaiDepartment of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI 48109, USA.
Alex DahlkemperDepartment of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI 48109, USA.
Darnell KaiglerDepartment of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI 48109, USA.ORCID 0000-0003-3728-4310
James L SherleyAsymmetrex® LLC, Boston, MA 02130, USA.ORCID 0000-0002-9711-3751
University of Michigan · USUniversity of Massachusetts Amherst · US

Funding

Mechanisms of dental pulp stem cell differentiation into functional endotheliumR01DE021410 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI NOR, JACQUES EDUARDO · 2011 to 2021
$3.2M
Redefining mesenchymal stem cells: using their cellular and molecular phenotypes to determine their regenerative and therapeutic propertiesR01DE028657 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI KAIGLER, DARNELL · 2019 to 2023
$2.1M
Regulation of dental pulp stem cell fateR37DE021410 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Jacques Eduardo Nor · 2024 to 2026
$1.2M
Increased Validation of a First Method for Counting Tissue Stem Cells SpecificallyR43HL154900 · NHLBI · ASYMMETREX, LLC · PI SHERLEY, JAMES L · 2020 to 2020
$420k
NHLBI NIH HHS R43 HL154900NIDCR NIH HHS R01 DE021410NIDCR NIH HHS R01 DE028657NIDCR NIH HHS R01DE028657NIDCR NIH HHS R37 DE021410NIDCR NIH HHS R56DE21410
6 · The paper itself

Abstract

Kinetic stem cell (KSC) counting is a recently introduced first technology for quantifying tissue stem cells in vertebrate organ and tissue cell preparations. Previously, effective quantification of the fraction or dosage of tissue stem cells had been largely lacking in stem cell science and medicine. A general method for the quantification of tissue stem cells will accelerate progress in both of these disciplines as well as related industries like drug development. Triplicate samples of human oral alveolar bone cell preparations, which contain mesenchymal stem cells (MSCs), were used to estimate the precision of KSC counting analyses conducted at three independent sites. A high degree of intra-site precision was found, with coefficients of variation for determinations of MSC-specific fractions of 8.9% (

Indexed as

alveolar bonecell therapykinetic stem cell countingmesenchymal stem cellprecisionrapid counting algorithmstem cell fractiontissue cell kineticstissue stem cellvariation

Identifiers

PMID38255666
PMCPMC10820168
OpenAlexW4390338865

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.