ArticleAnimals : an open access journal from MDPI2024
Establishment of an Indirect ELISA Method for the Detection of the Bovine Rotavirus VP6 Protein.
Article in Animals : an open access journal from MDPI, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 11 citations in OpenAlex.
- Evaluation of synthetic peptides derived from glycoproteins gp45 and gp90 as candidates for the immunodiagnosis of equine infectious anemia.Veterinary research communications · 2026Article
- Development of a Highly Sensitive ELISA for Detecting Antibodies Against a Novel Variant Avian Reovirus Based on Dual σC and σB Antigens.Animals : an open access journal from MDPI · 2026Article
- Development of MoSLife (Basel, Switzerland) · 2026Article
- A Monoclonal Antibody-Based Indirect Competitive ELISA for Detecting Goose Astrovirus Antibodies.Veterinary sciences · 2026Article
- Development and evaluation of an indirect ELISA for detecting ovine rotavirus antibodies based on VP6 protein.BMC veterinary research · 2026Article
- Isolation, Identification, and Comprehensive Genomic Characterization of a Bovine Rotavirus G10P[11] Strain in China.Polish journal of microbiology · 2025Article
- Establishment of an indirect ELISA method for detecting bovine coronavirus antibodies based on N protein.Frontiers in veterinary science · 2025Article
- Develop an indirect ELISA utilizing gD protein to detect antibodies against bovine herpesvirus type 1.Frontiers in cellular and infection microbiology · 2025Article
- Isolation, identification of porcine a rotavirus, and preparation of the monoclonal antibodies.Frontiers in veterinary science · 2025Article
- Diagnosis of bovine rotavirus: an overview of currently available methods.Frontiers in microbiology · 2025Review
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The objective of this study was to develop an indirect ELISA utilizing a polyclonal antibody against bovine rotavirus (BRV) VP6 protein. To achieve this, pcDNA3.1-VP6, a recombinant eukaryotic expression plasmid, was constructed based on the sequence of the conserved BRV gene VP6 and was transfected into CHO-K1 cells using the transient transfection method. The VP6 protein was purified as the coating antigen using nickel ion affinity chromatography, and an indirect ELISA was subsequently established. The study found that the optimal concentration of coating for the VP6 protein was 1 μg/mL. The optimal blocking solution was 3% skim milk, and the blocking time was 120 min. The secondary antibody was diluted to 1:4000, and the incubation time for the secondary antibody was 30 min. A positive result was indicated when the serum OD450 was greater than or equal to 0.357. The coefficients of variation were less than 10% both within and between batches, indicating the good reproducibility of the method. The study found that the test result was positive when the serum dilution was 2
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