Evidence map›Paper›PMID 38252343›Full record

ArticleMolecular biology reports2024

Cloning, tissue expression and imprinting status analysis of the NDN gene in Dolang sheep.

Yongjie Zhang, Zhiyuan Sui, Zhishuai Zhang, Chenguang Wang, Xiaojun Li, Feng Xing

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Yongjie ZhangCollege of Animal Science and Technology, Tarim University, Alar, Xinjiang, China.
Zhiyuan SuiCollege of Animal Science and Technology, Tarim University, Alar, Xinjiang, China.
Zhishuai ZhangCollege of Animal Science and Technology, Tarim University, Alar, Xinjiang, China.
Chenguang WangCollege of Animal Science and Technology, Tarim University, Alar, Xinjiang, China.
Xiaojun LiCollege of Animal Science and Technology, Tarim University, Alar, Xinjiang, China.
Feng XingCollege of Animal Science and Technology, Tarim University, Alar, Xinjiang, China. 19882954983@163.com.
Tarim University · CNXinjiang Production and Construction Corps · CN

Funding

National Natural Science Foundation of China 31660652
6 · The paper itself

Abstract

backgroundGenomic imprinting refers to expressing parent-specific genes in mammalian diploid cells. The NDN gene is maternally imprinted in humans and mice and correlates with the timing of puberty. This study aimed to investigate its imprinting status and its relationship with the onset of puberty in Dolang sheep. METHODS AND

resultsIn this study, cloning and sequencing obtained the NDN gene cDNA sequence of 1082 bp of Dolang sheep, coding for 325 amino acids. Similarity analysis and phylogenetic tree showed that the NDN gene conformed to the law of speciation and was highly conserved among mammals. RT-qPCR results showed the highest expression of NDN mRNA was found in the hypothalamus at puberty, and the expression was significantly increased and then significantly decreased from prepuberty to postpuberty in the hypothalamus, pituitary, and ovary and oviduct. Based on expressed single nucleotide polymorphism (SNP), the NDN gene was expressed monoallelically in the tissues of adult and neonatal umbilical cords, and the expressed allele was paternally inherited. The NDN promoter region of 3400 bp was obtained by cloning and identified in monoallelic-expressing tissues (hypothalamus, ovary, spleen) as a differentially methylated region (DMR).

conclusionThese findings will enrich the number of imprinted genes in sheep and suggest that the NDN gene could be a candidate gene for studying puberty initiation in Dolang sheep.

Indexed as

Amino AcidsGenes, cdcAllelesAnimalsCloning, MolecularFemalePhylogenySheepAmino AcidsDNA methylationDolang sheepImprinted geneNDN geneTissue expression

Identifiers

PMID38252343
OpenAlexW4391109694

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.