Evidence map›Paper›PMID 38251787›Full record

ArticleJournal of visualized experiments : JoVE2024

4D Light-sheet Imaging of Zebrafish Cardiac Contraction.

Xinyuan Zhang, Alireza Saberigarakani, Milad Almasian, Sohail Hassan, Manasa Nekkanti, Yichen Ding

Open access · greenAbstract readVideo-Audio Media
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of visualized experiments : JoVE, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Light-Sheet Imaging to Reveal Cardiac Structure in Rodent Hearts.Journal of visualized experiments : JoVE · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Xinyuan ZhangDepartment of Bioengineering, The University of Texas at Dallas.
Alireza SaberigarakaniDepartment of Bioengineering, The University of Texas at Dallas.
Milad AlmasianDepartment of Bioengineering, The University of Texas at Dallas.
Sohail HassanDepartment of Bioengineering, The University of Texas at Dallas.
Manasa NekkantiDepartment of Bioengineering, The University of Texas at Dallas.
Yichen DingDepartment of Bioengineering, The University of Texas at Dallas; Center for Imaging and Surgical Innovation, The University of Texas at Dallas; Hamon Center for Regenerative Science and Medicine, UT Southwestern Medical Center; Yichen.Ding@utdallas.edu.
The University of Texas at Dallas · USSouthwestern Medical Center

Funding

Volumetric imaging and computation to characterize cardiac electromechanical couplingR01HL162635 · NHLBI · UNIVERSITY OF TEXAS DALLAS · PI Yichen Ding · 2023 to 2026
$1.5M
Integrating imaging and computation to characterize neural crest cells in the myocardial development and regenerationR00HL148493 · NHLBI · UNIVERSITY OF TEXAS DALLAS · PI DING, YICHEN · 2020 to 2022
$730k
NHLBI NIH HHS R00 HL148493NHLBI NIH HHS R01 HL162635
6 · The paper itself

Abstract

Zebrafish is an intriguing model organism known for its remarkable cardiac regeneration capacity. Studying the contracting heart in vivo is essential for gaining insights into structural and functional changes in response to injuries. However, obtaining high-resolution and high-speed 4-dimensional (4D, 3D spatial + 1D temporal) images of the zebrafish heart to assess cardiac architecture and contractility remains challenging. In this context, an in-house light-sheet microscope (LSM) and customized computational analysis are used to overcome these technical limitations. This strategy, involving LSM system construction, retrospective synchronization, single cell tracking, and user-directed analysis, enables one to investigate the micro-structure and contractile function across the entire heart at the single-cell resolution in the transgenic Tg(myl7:nucGFP) zebrafish larvae. Additionally, we are able to further incorporate microinjection of small molecule compounds to induce cardiac injury in a precise and controlled manner. Overall, this framework allows one to track physiological and pathophysiological changes, as well as the regional mechanics at the single-cell level during cardiac morphogenesis and regeneration.

Indexed as

Muscle ContractionZebrafishAnimalsAnimals, Genetically ModifiedCell TrackingRetrospective Studies

Identifiers

PMID38251787
PMCPMC10939705
OpenAlexW4390631017

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.