Evidence map›Paper›PMID 38247823›Full record

ArticleCells2024

Modeling of Retina and Optic Nerve Ischemia-Reperfusion Injury through Hypoxia-Reoxygenation in Human Induced Pluripotent Stem Cell-Derived Retinal Ganglion Cells.

Tomoyo Yoshida, Tadashi Yokoi, Taku Tanaka, Emiko Matsuzaka, Yuki Saida, Sachiko Nishina, Shuji Takada, Shigeomi Shimizu, Noriyuki Azuma

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Tomoyo YoshidaNational Center for Child Health and Development, 2-10-1, O-kura, Setagaya-ku, Tokyo 1578535, Japan.
Tadashi YokoiNational Center for Child Health and Development, 2-10-1, O-kura, Setagaya-ku, Tokyo 1578535, Japan.
Taku TanakaNational Center for Child Health and Development, 2-10-1, O-kura, Setagaya-ku, Tokyo 1578535, Japan.
Emiko MatsuzakaNational Center for Child Health and Development, 2-10-1, O-kura, Setagaya-ku, Tokyo 1578535, Japan.
Yuki SaidaNational Center for Child Health and Development, 2-10-1, O-kura, Setagaya-ku, Tokyo 1578535, Japan.
Sachiko NishinaNational Center for Child Health and Development, 2-10-1, O-kura, Setagaya-ku, Tokyo 1578535, Japan.
Shuji TakadaNational Center for Child Health and Development, 2-10-1, O-kura, Setagaya-ku, Tokyo 1578535, Japan.
Shigeomi ShimizuDepartment of Pathological Cell Biology, Tokyo Medical and Dental University, 1-5-4, Yushima, Bunkyo-ku, Tokyo 1138510, Japan.ORCID 0000-0002-8437-0791
Noriyuki AzumaNational Center for Child Health and Development, 2-10-1, O-kura, Setagaya-ku, Tokyo 1578535, Japan.
National Center For Child Health and Development · JPTokyo Medical and Dental University · JPKyorin University · JP

Funding

JSPS KAKENHI 22390327JSPS KAKENHI 24659770National Center for Child Health and Development 2020B-5National Center for Child Health and Development 28-2
6 · The paper itself

Abstract

Retinal ganglion cells (RGCs) are specialized projection neurons that constitute part of the retina, and the death of RGCs causes various eye diseases, but the mechanism of RGC death is still unclear. Here, we induced cell death in human induced pluripotent stem cell (hiPSC)-derived RGC-rich retinal tissues using hypoxia-reoxygenation in vitro. Flow cytometry, immunochemistry, and Western blotting showed the apoptosis and necrosis of RGCs under hypoxia-reoxygenation, and they were rescued by an apoptosis inhibitor but not by a necrosis inhibitor. This revealed that the cell death induced in our model was mainly due to apoptosis. To our knowledge, this is the first model to reproduce ischemia-reperfusion in hiPSC-derived RGCs. Thus, the efficacy of apoptosis inhibitors and neuroprotective agents can be evaluated using this model, bringing us closer to clinical applications.

Indexed as

Induced Pluripotent Stem CellsOptic Neuropathy, IschemicReperfusion InjuryHumansHypoxiaNecrosisOptic NerveRetinaRetinal Ganglion Cellsapoptosishuman iPS cellshypoxia–reoxygenationischemia–reperfusionretinal ganglion cells

Identifiers

PMID38247823
PMCPMC10814087
OpenAlexW4390747527

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.