Evidence map›Paper›PMID 38245573›Full record

ArticleAMB Express2024

Improving the thermostability of Pseudoalteromonas Porphyrae κ-carrageenase by rational design and MD simulation.

Yuyan Sang, Xiaoyi Huang, Hebin Li, Tao Hong, Mingjing Zheng, Zhipeng Li, Zedong Jiang, Hui Ni, Qingbiao Li, Yanbing Zhu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in AMB Express, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yuyan SangCollege of Ocean Food and Biological Engineering, Jimei University, 361021, Xiamen, China.
Xiaoyi HuangCollege of Ocean Food and Biological Engineering, Jimei University, 361021, Xiamen, China.
Hebin LiDepartment of Pharmacy, Xiamen Medical College, 361008, Xiamen, China.
Tao HongCollege of Ocean Food and Biological Engineering, Jimei University, 361021, Xiamen, China.
Mingjing ZhengCollege of Ocean Food and Biological Engineering, Jimei University, 361021, Xiamen, China.
Zhipeng LiCollege of Ocean Food and Biological Engineering, Jimei University, 361021, Xiamen, China.
Zedong JiangCollege of Ocean Food and Biological Engineering, Jimei University, 361021, Xiamen, China.
Hui NiCollege of Ocean Food and Biological Engineering, Jimei University, 361021, Xiamen, China.
Qingbiao LiCollege of Ocean Food and Biological Engineering, Jimei University, 361021, Xiamen, China.
Yanbing ZhuCollege of Ocean Food and Biological Engineering, Jimei University, 361021, Xiamen, China. yanbingzhu@jmu.edu.cn.ORCID http://orcid.org/0000-0003-2771-1235

Funding

the National Natural Science Foundation of China 22178142the National Natural Science Foundation of China 41976124the Natural Science Foundation of Fujian Province of China 2023J011652
6 · The paper itself

Abstract

The industrial applications of the κ-carrageenases have been restricted by their poor thermostability. In this study, based on the folding free energy change (ΔΔG) and the flexibility analysis using molecular dynamics (MD) simulation for the alkaline κ-carrageenase KCgCD from Pseudoalteromonas porphyrae (WT), the mutant S190R was identified with improved thermostability. After incubation at 50 °C for 30 min, the residual activity of S190R was 63.7%, 25.7% higher than that of WT. The T

Indexed as

Folding free energy changeMolecular dynamic simulationMutant κ-carrageenaseThermostability improvement

Identifiers

PMID38245573
PMCPMC10799840

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.