Evidence map›Paper›PMID 38244177›Full record

ArticleMolecular biotechnology2024

Tobacco Plant: A Novel and Promising Heterologous Bioreactor for the Production of Recombinant Bovine Chymosin.

Shahnam Azizi-Dargahlou, Mahin Pouresmaeil, Mohammad Ahmadabadi

Abstract read
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Article in Molecular biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.9field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 3 institutions in 1 country.

Shahnam Azizi-DargahlouSeed and Plant Certification and Registration Institute, Ardabil Agricultural and Natural Resources Research Center, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. shahnambio@gmail.com.ORCID http://orcid.org/0000-0002-7658-5135
Mahin PouresmaeilFaculty of Agricultural Sciences and Natural Resources, University of Mohaghegh Ardabili, Ardabi, Iran.
Mohammad AhmadabadiDepartment of Biotechnology, Azarbaijan Shahid Madani University, Tabriz, Iran.
Agricultural Research & Education Organization · IRAzarbaijan Shahid Madani University · IRUniversity of Mohaghegh Ardabili · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The natural source of chymosin, a key enzyme in the dairy industry, is insufficient for rapidly growing cheese industries. Large-scale production of recombinant proteins in heterologous hosts provides an efficient alternative solution. Here, the codon-optimized synthetic prochymosin gene, which has a CAI index of 0.926, was subcloned from a cloning vector (pUC57-bCYM) into the pBI121 vector, resulting in the construct named pBI121-bCYM. CAI ranges from 0 to 1 and higher CAI improves gene expression in heterologous hosts. The overexpression of the prochymosin gene was under the control of constitutive CaMV 35S promoter and NOS terminator and was transferred into the tobacco via A. tumefaciens strain LBA4404. Explant type, regeneration method, inoculation temperature, cell density (OD

Indexed as

BioreactorsChymosinNicotianaPlants, Genetically ModifiedRecombinant ProteinsAgrobacterium tumefaciensAnimalsCattleGenetic VectorsChymosinRecombinant ProteinsCodon Adaptation Index (CAI)Codon-optimized prochymosinHeterologous hostNicotiana tabacumRecombinant chymosin

Identifiers

PMID38244177
OpenAlexW4391052460

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.