Evidence map›Paper›PMID 38243925›Full record

ArticleProtein and peptide letters2024

Feasibility of Domain Segmentation of B19V VP1u Using Intein Technology for Structural Studies.

Renuk Varayil Lakshmanan, Mavis Agbandje-McKenna, Robert McKenna

Abstract read
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In one paragraph

Article in Protein and peptide letters, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Renuk Varayil LakshmananDepartment of Biochemistry and Molecular Biology, College of Medicine, Center for Structural Biology, McKnight Brain Institute, University of Florida, USA.
Mavis Agbandje-McKennaDepartment of Biochemistry and Molecular Biology, College of Medicine, Center for Structural Biology, McKnight Brain Institute, University of Florida, USA.
Robert McKennaDepartment of Biochemistry and Molecular Biology, College of Medicine, Center for Structural Biology, McKnight Brain Institute, University of Florida, USA.
University of Florida · USAllen Institute for Brain Science · US

Funding

National Institute of Health Grant Number GM082946
6 · The paper itself

Abstract

introductionParvovirus B19 (B19V) is a human pathogen, and the minor capsid protein of B19V possesses a unique N terminus called VP1u that plays a crucial role in the life cycle of the virus.

objectivesThe objective of this study was to develop a method for domain segmentation of B19 VP1u using intein technology, particularly its receptor binding domain (RBD) and phospholipase A2 (PLA

methodsRBD and PLA

resultsThe CD spectrum of the full length VP1u confirmed the secondary structure of protein, while the PLA

conclusionThis method would allow for the selective incorporation of NMR-active isotopes into either of the VP1u domains, which can reduce signal overlap in NMR structural determination studies.

Indexed as

Capsid ProteinsEscherichia coliInteinsCircular DichroismHumansNostocParvovirus B19, HumanPhospholipases A2Protein DomainsCapsid ProteinsPhospholipases A2B19VCD spectroscopy.inteinsParvovirusesphospholipaseVP1u

Identifiers

PMID38243925
OpenAlexW4391067280

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.