Evidence map›Paper›PMID 38243767›Full record

Observational studyMilitary medicine2024

Dynamics of the Oral Microbiome During Initial Military Training at Fort Benning, Georgia.

Kristina K Zudock, Robert Player, Amanda Ernlund, Collin M Timm, Caroline E English, Michael W Ellis, David R Tribble, D Scott Merrell, Jason W Bennett, Eugene V Millar

Erratum issuedOpen access · greenAbstract readObservational Study
PubMed Full text at PMC Publisher
In one paragraph

Observational study in Military medicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Kristina K ZudockResearch and Exploratory Development Department, Johns Hopkins University Applied Physics Laboratory, Laurel, MD 20723, USA.ORCID 0000-0002-7968-1245
Robert PlayerAsymmetric Operations Sector, Johns Hopkins University Applied Physics Laboratory, Laurel, MD 20723, USA.ORCID 0000-0001-5872-259X
Amanda ErnlundResearch and Exploratory Development Department, Johns Hopkins University Applied Physics Laboratory, Laurel, MD 20723, USA.ORCID 0000-0003-1113-4103
Collin M TimmAsymmetric Operations Sector, Johns Hopkins University Applied Physics Laboratory, Laurel, MD 20723, USA.ORCID 0000-0002-7804-5044
Caroline E EnglishInfectious Disease Clinical Research Program, Department of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.
Michael W EllisDivision of Infectious Diseases, University of Toledo College of Medicine and Life Sciences, Toledo, OH 43614, USA.
David R TribbleInfectious Disease Clinical Research Program, Department of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.
D Scott MerrellDepartment of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.ORCID 0000-0001-7095-5177
Jason W BennettMultidrug-Resistant Organism Repository and Surveillance Network, Bacterial Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA.
Eugene V MillarInfectious Disease Clinical Research Program, Department of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.ORCID 0000-0002-2019-6480
Johns Hopkins University Applied Physics Laboratory · USUniformed Services University of the Health Sciences · USHenry M. Jackson Foundation · USUniversity of Toledo · US

Funding

DATA CENTER FOR EPIDEMIOLOGIC INVESTIGATIONS OF HIVN01AI005072 · NIAID · NEW ENGLAND RESEARCH INSTITUTES, INC. · PI MCKINLAY, SONJA M · 1990 to 1994
–
Defense Health Agency HU0001190002Division of Intramural Research, National Institute of Allergy and Infectious Diseases IAA Y1-Al-5072
6 · The paper itself

Abstract

introductionMilitary trainees are at increased risk for infectious disease outbreaks because of the unique circumstances of the training environment (e.g., close proximity areas and physiologic/psychologic stress). Standard medical countermeasures in military training settings include routine immunization (e.g., influenza and adenovirus) as well as chemoprophylaxis [e.g., benzathine penicillin G (Bicillin) for the prevention of group A streptococcal disease] for pathogens associated with outbreaks in these settings. In a population of U.S. Army Infantry trainees, we evaluated changes in the oral microbiome during a 14-week military training cycle. MATERIALS AND

methodsTrainees were enrolled in an observational cohort study in 2015-2016. In 2015, Bicillin was administered to trainees to ameliorate the risk of group A Streptococcus outbreaks, whereas in 2016, trainees did not receive a Bicillin inoculation. Oropharyngeal swabs were collected from participants at days 0, 7, 14, 28, 56, and 90 of training. Swabs were collected, flash frozen, and stored. DNA was extracted from swabs, and amplicon sequencing of the 16s rRNA gene was performed. Microbiome dynamics were evaluated using the QIIME 2 workflow along with DADA2, SINA with SILVA, and an additional processing in R.

resultsWe observed that microbiome samples from the baseline (day 0) visit were distinct from one another, whereas samples collected on day 14 exhibited significant microbiome convergence. Day 14 convergence was coincident with an increase in DNA sequences associated with Streptococcus, though there was not a significant difference between Streptococcus abundance over time between 2015 and 2016 (P = .07), suggesting that Bicillin prophylaxis did not significantly impact overall Streptococcus abundance.

conclusionsThe temporary convergence of microbiomes is coincident with a rise in communicable infections in this population. The dynamic response of microbiomes during initial military training supports similar observations in the literature of transient convergence of the human microbiome under cohabitation in the time frame including in this experiment. This population and the associated longitudinal studies allow for controlled studies of human microbiome under diverse conditions.

Indexed as

MicrobiotaMilitary PersonnelCohort StudiesFemaleGeorgiaHumansMaleMouthRNA, Ribosomal, 16SRNA, Ribosomal, 16S

Identifiers

PMID38243767
PMCPMC11221557
OpenAlexW4391053637

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.