ArticlePlant communications2024
An inducible CRISPR activation tool for accelerating plant regeneration.
Article in Plant communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Tissue-specific recombination-activated clonal and inducible transcription (TRAC-IT) enables controlled gene activation in stably marked tissues.The Plant journal : for cell and molecular biology · 2026Article
- Dexamethasone-inducible LhGR/pOp system: simple and flexible spatiotemporal control of gene expression.Planta · 2026Review
- Boosting genome editing in perennial plants by CRISPR-Combo mediated morphogenic gene activation.Nature communications · 2026Article
- Plant Regeneration: Influencing Factors, Regulatory Networks, and Epigenetic Mechanisms.International journal of molecular sciences · 2026Review
- Beyond Starch: Towards a Scalable Potato Platform for Molecular Farming.Plant biotechnology journal · 2026Review
- Tuning Xylan Polymerisation Enhanced Fibre Digestibility Without Biomass Loss in Sheepgrass (Leymus chinensis).Plant biotechnology journal · 2026Article
- Overcoming breeding barriers with genome editing in autopolyploid crops.BMC plant biology · 2026Review
- Exploring the developmental mechanisms of tea plant trichomes using genomics and single-cell transcriptome sequencing.Horticulture research · 2026Article
- The emerging impact of CRISPR and gene editing on global crop improvement.Transgenic research · 2026Review
- Advances in the use of morphogenic regulators and peptide regenerating factors for boosting plant transformation and genome editing.Frontiers in plant science · 2026Review
- Research progress and applications of gene activation editing technology in crops.Frontiers in plant science · 2026Review
- Engineering Conditional Transgene Expression in Nicotiana benthamiana.Plant biotechnology journal · 2026Review
- Why "Where" Matters as Much as "How Much": Single-Cell and Spatial Transcriptomics in Plants.International journal of molecular sciences · 2025Review
- CRISPR targeting of H3K4me3 activates gene expression and unlocks centromere-proximal crossover recombination in Arabidopsis.Nature communications · 2025Article
- Turbo-charging crop improvement: harnessing multiplex editing for polygenic trait engineering and beyond.The Plant journal : for cell and molecular biology · 2025Review
- Co-transformation using T-DNA genes from Agrobacterium strain 82.139 enhances regeneration of transgenic shoots in Populus.Plant biotechnology journal · 2025Article
- Programmable genome engineering and gene modifications for plant biodesign.Plant communications · 2025Review
- Forage Crop Research in the Modern Age.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Epigenetics in the modern era of crop improvements.Science China. Life sciences · 2025Review
- Harnessing promoter elements to enhance gene editing in plants: perspectives and advances.Plant biotechnology journal · 2025Review
Corrections and comments
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Authors and funding
18 authors.
Funding
Abstract
The inducible CRISPR activation (CRISPR-a) system offers unparalleled precision and versatility for regulating endogenous genes, making it highly sought after in plant research. In this study, we developed a chemically inducible CRISPR-a tool for plants called ER-Tag by combining the LexA-VP16-ER inducible system with the SunTag CRISPR-a system. We systematically compared different induction strategies and achieved high efficiency in target gene activation. We demonstrated that guide RNAs can be multiplexed and pooled for large-scale screening of effective morphogenic genes and gene pairs involved in plant regeneration. Further experiments showed that induced activation of these morphogenic genes can accelerate regeneration and improve regeneration efficiency in both eudicot and monocot plants, including alfalfa, woodland strawberry, and sheepgrass. Our study expands the CRISPR toolset in plants and provides a powerful new strategy for studying gene function when constitutive expression is not feasible or ideal.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.