ArticleScientific reports2024
Drying as an effective method to store soil samples for DNA-based microbial community analyses: a comparative study.
Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 15 citations in OpenAlex.
- Rhizosphere and Soil Depth Differentially Shape Microbial Community Composition and Assembly inMicroorganisms · 2026Article
- Metagenomic Insights Into Red Sea Biodiversity Across the Web of Life.Environmental microbiology · 2026Article
- Assessment of Stability of Preservation Techniques for Strawberry Soil Microbiomes: Exploring the Biotechnological Relevance for Sustainable Agriculture.Microorganisms · 2026Article
- A fixed methane filter maximizes freshwater emissions under warming.Nature climate change · 2026Article
- Type I Sourdough Preservation Strategies and the Contribution of Microbial Biological Resource Centers to Biodiversity Protection: A Narrative Review.Foods (Basel, Switzerland) · 2025Review
- Nitrogen cycling genes abundance in soil and aboveground compartments of tropical peatland cloud forests and a wetland on Réunion Island.Scientific reports · 2025Article
- Desiccation as a suitable alternative to cold-storage of phyllosphere samples for DNA-based microbial community analyses.Scientific reports · 2025Article
- Soil storage temperature and air-drying did not significantly change bacterial taxa in the short-term.PeerJ · 2025Article
- Strengthening the Korean Network of Microbial Culture Collections in the Microbiome Era.Mycobiology · 2024Review
- Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Soil sampling for environmental DNA in remote and semi-remote locations is often limited due to logistical constraints surrounding sample preservation, including no or limited access to a freezer. Freezing at - 20 °C is a common DNA preservation strategy, however, other methods such as desiccation, ethanol or commercial preservatives are available as potential alternative DNA preservation methods for room temperature storage. In this study, we assessed five preservation methods (CD1 solution, 95% Ethanol, Dry & Dry silica gel packs, RNAlater, LifeGuard) along with freezing at - 20 °C, against immediate extraction on organic and mineral soils for up to three weeks of preservation. We assessed direct effects on DNA concentration and quality, and used DNA metabarcoding to assess effects on bacterial and fungal communities. Drying with Dry & Dry led to no significant differences from immediate extraction. RNAlater led to lower DNA concentrations, but effects on community structures were comparable to freezing. CD1, LifeGuard and Ethanol either caused immediate significant shifts in community structure, degradation of DNA quality or changes in diversity metrics. Overall, our study supports the use of drying with silica gel packs as a cost-effective, and easily applied method for the short-term storage at room temperature for DNA-based microbial community analyses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.