Evidence map›Paper›PMID 38242343›Full record

ArticleDevelopmental biology2024

ARF6, a component of intercellular bridges, is essential for spermatogenesis in mice.

Hetty N Wong, Tingfang Chen, P Jeremy Wang, Lawrence B Holzman

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.7field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Hetty N WongRenal, Electrolyte, and Hypertension Division, Department of Medicine, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA, 19104, USA.
Tingfang ChenDepartment of Genetics, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA, 19104, USA.
P Jeremy WangDepartment of Biomedical Sciences, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA, 19104, USA.
Lawrence B HolzmanRenal, Electrolyte, and Hypertension Division, Department of Medicine, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA, 19104, USA. Electronic address: lholzman@pennmedicine.upenn.edu.
University of Pennsylvania · US

Funding

Slit Diaphragm and Actin DynamicsR01DK080751 · NIDDK · UNIVERSITY OF PENNSYLVANIA · PI HOLZMAN, LAWRENCE B. · 2008 to 2017
$3.6M
Role of TET Proteins in ES Cell Pluripotency and FunctionR01HD065812 · NICHD · LA JOLLA INSTITUTE FOR IMMUNOLOGY · PI RAO, ANJANA · 2011 to 2015
$1.8M
NICHD NIH HHS R01 HD065812NIDDK NIH HHS R01 DK080751
6 · The paper itself

Abstract

Male germ cells are connected by intercellular bridges (ICBs) in a syncytium due to incomplete cytokinesis. Syncytium is thought to be important for synchronized germ cell development by interchange of cytoplasmic factors via ICBs. Mammalian ADP-ribosylation factor 6 (ARF6) is a small GTPase that is involved in many cellular mechanisms including but not limited to regulating cellular structure, motility, vesicle trafficking and cytokinesis. ARF6 localizes to ICBs in spermatogonia and spermatocytes in mice. Here we report that mice with global depletion of ARF6 in adulthood using Ubc-CreERT2 display no observable phenotypes but are male sterile. ARF6-deficient males display a progressive loss of germ cells, including LIN28A-expressing spermatogonia, and ultimately develop Sertoli-cell-only syndrome. Specifically, intercellular bridges are lost in ARF6-deficient testis. Furthermore, germ cell-specific inactivation using the Ddx4-CreERT2 results in the same testicular morphological phenotype, showing the germ cell-intrinsic requirement of ARF6. Therefore, ARF6 is essential for spermatogenesis in mice and this function is conserved from Drosophila to mammals.

Indexed as

ADP-Ribosylation Factor 6SpermatogenesisActivating Transcription Factor 6AnimalsDrosophilaFemaleMaleMammalsMiceSpermatocytesSpermatogoniaTestisActivating Transcription Factor 6ADP-Ribosylation Factor 6Atf6 protein, mouseARF6Intercellular bridgesMale infertilitySpermatogenesis

Identifiers

PMID38242343
PMCPMC10979378
OpenAlexW4391056089

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.