Evidence map›Paper›PMID 38240803›Full record

ArticleApplied microbiology and biotechnology2024

CRISPR/Cas9 system is a suitable gene targeting editing tool to filamentous fungus Monascus pilosus.

Yunxia Gong, Shengfa Li, Qianrui Liu, Fusheng Chen, Yanchun Shao

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
3.5field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Advances in CRISPR/Cas9-Based Gene Editing in Filamentous Fungi.Journal of fungi (Basel, Switzerland) · 2025
    Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Yunxia GongCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
Shengfa LiCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
Qianrui LiuCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
Fusheng ChenCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
Yanchun ShaoCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. yanchunshao@mail.hzau.edu.cn.ORCID http://orcid.org/0000-0001-6755-8023
Huazhong Agricultural University · CN

Funding

National Natural Science Foundation of China 31671835National Natural Science Foundation of China 32272288
6 · The paper itself

Abstract

Monascus pilosus has been used to produce lipid-lowering drugs rich in monacolin K (MK) for a long period. Genome mining reveals there are still many potential genes worth to be explored in this fungus. Thereby, efficient genetic manipulation tools will greatly accelerate this progress. In this study, we firstly developed the protocol to prepare protoplasts for recipient of CRISPR/Cas9 system. Subsequently, the vector and donor DNA were co-transformed into recipients (10

Indexed as

Gene EditingMonascusAgrobacterium tumefaciensCRISPR-Cas SystemsDNAGene TargetingLovastatinDNALovastatinAgrobacterium tumefaciens-mediated transformationCRISPR/Cas9 systemDNA damage responseGene replacement frequencyMK-producing abilityMonascus species

Identifiers

PMID38240803
PMCPMC10799099
OpenAlexW4391034728

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.