ArticleMicrobial genomics2024
Tracking arboviruses, their transmission vectors and potential hosts by nanopore sequencing of mosquitoes.
Article in Microbial genomics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Assessing the Use of Oxford Nanopore Technologies Sequencing for Detection of Stable Wolbachia Bacterial Strain in Symbiosis in Wild Anopheles Mosquitoes From the WHO African Region.Environmental microbiology · 2026Article
- A multiplex assay to detect mosquito species, bloodmeal host source and Plasmodium in malaria vectors using Nanopore amplicon sequencing.Medical and veterinary entomology · 2026Article
- First molecular detection of the genus Almendravirus in Johnbelkinia ulopus and Anopheles apicimacula mosquitoes from the Colombian Caribbean.Parasites & vectors · 2026Article
- Evaluating the Effect of Sampling Scale on Mosquito Virome Characterization Using PacBio HiFi Long-Read Metagenomics.Insects · 2026Article
- Blood-feeding Patterns of Amazonian Forest Edge Mosquitoes.Research square · 2026Article
- Real-time genomic pathogen, resistance, and host range characterization from passive water sampling of wetland ecosystems.Applied and environmental microbiology · 2026Article
- Evolution and spillover dynamics of yellow fever at the forest-urban interface in Brazil.Nature microbiology · 2026Article
- RNA virome comparison between sylvatic and urban-interface mosquitoes from Southeastern Brazil.Frontiers in cellular and infection microbiology · 2026Article
- Characterizing the Bacterial Microbiome of the Invasive VectorInternational journal of microbiology · 2026Article
- Viral Metagenomic Next-Generation Sequencing for One Health Discovery and Surveillance of (Re)Emerging Viruses: A Deep Review.International journal of molecular sciences · 2025Review
- Viruses in Simuliidae: An Updated Systematic Review of Arboviral Diversity and Vector Potential.Life (Basel, Switzerland) · 2025Review
- Isopods infesting Atlantic bonefish (Parasitology · 2024Article
- Exploring mosquito virome dynamics within São Paulo Zoo: insights into mosquito-virus-environment interactions.Frontiers in cellular and infection microbiology · 2024Article
Corrections and comments
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Authors and funding
14 authors.
Funding
Abstract
The risk to human health from mosquito-borne viruses such as dengue, chikungunya and yellow fever is increasing due to increased human expansion, deforestation and climate change. To anticipate and predict the spread and transmission of mosquito-borne viruses, a better understanding of the transmission cycle in mosquito populations is needed. We present a pathogen-agnostic combined sequencing protocol for identifying vectors, viral pathogens and their hosts or reservoirs using portable Oxford Nanopore sequencing. Using mosquitoes collected in São Paulo, Brazil, we extracted RNA for virus identification and DNA for blood meal and mosquito identification. Mosquitoes and blood meals were identified by comparing cytochrome c oxidase I (COI) sequences against a curated Barcode of Life Data System (BOLD). Viruses were identified using the SMART-9N protocol, which allows amplified DNA to be prepared with native barcoding for nanopore sequencing. Kraken 2 was employed to detect viral pathogens and Minimap2 and BOLD identified the contents of the blood meal. Due to the high similarity of some species, mosquito identification was conducted using blast after generation of consensus COI sequences using RACON polishing. This protocol can simultaneously uncover viral diversity, mosquito species and mosquito feeding habits. It also has the potential to increase understanding of mosquito genetic diversity and transmission dynamics of zoonotic mosquito-borne viruses.
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