Opsin-Free Activation of Bmp Receptors by a Femtosecond Laser.

Manjun Xu, Haipeng Wang, Xiaoying Tian, Bingyi Li, Shaoyang Wang, Xiaohui Zhao, Hao He

Abstract read

In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Opsin-Free Activation of Bmp Receptors by a Femtosecond Laser.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Manjun XuSchool of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200031, China.

Haipeng WangSchool of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200031, China.

Xiaoying TianSchool of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200031, China.

Bingyi LiSchool of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200031, China.

Shaoyang WangSchool of Biomedical Engineering, Hainan University, Haikou, 570228, China.

Xiaohui ZhaoSchool of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200031, China.

Hao HeSchool of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200031, China.ORCID 0000-0003-0832-8310

Funding

National Natural Science Foundation of China 61975118National Natural Science Foundation of China 62022056National Natural Science Foundation of China 92054105Shanghai Jiao Tong University YG2022QN063

6 · The paper itself

Abstract

Bone morphogenetic protein (BMP) signaling plays a vital role in differentiation, organogenesis, and various cell processes. As a member of TGF-β superfamily, the BMP initiation usually accompanies crosstalk with other signaling pathways and simultaneously activates some of them. It is quite challenging to solely initiate an individual pathway. In this study, an opsin-free optical method to specifically activate BMP receptors (BMPR) and subsequent pSmad1/5/8 cascades by a single-time scan of a tightly-focused femtosecond laser in the near infrared range is reported. Via transient two-photon excitation to intrinsic local flavins near the cell membrane, the photoactivation drives conformational changes of preformed BMPR complexes to enable their bonding and phosphorylation of the GS domain in BMPR-I by BMPR-II. The pSmad1/5/8 signaling is initiated by this method, while p38 and pSmad2 are rarely perturbed. Based on a microscopic system, primary adipose-derived stem cells in an area of 420 × 420 µm

Indexed as

Bone Morphogenetic Protein 2Bone Morphogenetic ProteinsBone Morphogenetic Protein ReceptorsOpsinsTransforming Growth Factor betaBone Morphogenetic Protein 2Bone Morphogenetic Protein ReceptorsBone Morphogenetic ProteinsOpsinsTransforming Growth Factor betaBMP receptorsconformational changeopsin-free activationsignaling pathwaystwo-photon excitation

Identifiers

PMID38239021

DOI10.1002/advs.202308072

PMCPMC10933676

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.