Evidence map›Paper›PMID 38238372›Full record

ArticleScientific reports2024

Metagenomic analysis of Mesolithic chewed pitch reveals poor oral health among stone age individuals.

Emrah Kırdök, Natalija Kashuba, Hege Damlien, Mikael A Manninen, Bengt Nordqvist, Anna Kjellström, Mattias Jakobsson, A Michael Lindberg, Jan Storå, Per Persson and 3 more

Abstract readCase Reports
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Emrah KırdökDepartment of Biotechnology, Faculty of Science, Mersin University, 33100 Yenişehir, Mersin, Turkey. emrahkirdok@gmail.com.
Natalija KashubaDepartment of Archaeology and Ancient History, Uppsala University, Engelska Parken, Thunbergsvägen 3H Box 626, 751 26, Uppsala, Sweden.
Hege DamlienMuseum of Cultural History, University of Oslo, St. Olavs Plass, P.O. Box 6762, NO-0130, Oslo, Norway.
Mikael A ManninenPAES, Ecosystems and Environment Research Programme, Faculty of Biological and Environmental Sciences and Helsinki Institute of Sustainability Science, University of Helsinki, Viikinkaari 1, P.O. Box 65, Helsinki, Finland.
Bengt NordqvistFoundation War-Booty Site Finnestorp, Klarinettvägen 75, 434 75, Kungsbacka, Sweden.
Anna KjellströmDepartment of Archaeology and Classical Studies, Osteoarchaeological Research Laboratory, Stockholm University, Stockholm, Sweden.
Mattias JakobssonDepartment of Organismal Biology, Human Evolution, Uppsala University, Evolutionsbiologiskt Centrum EBC Norbyvägen 18 A, Uppsala, Sweden.
A Michael LindbergDepartment of Chemistry and Biomedical Sciences, Faculty of Health and Life Sciences, Linnaeus University, Hus Vita, 44018, Kalmar, Sweden.
Jan StoråDepartment of Archaeology and Classical Studies, Osteoarchaeological Research Laboratory, Stockholm University, Stockholm, Sweden.
Per PerssonMuseum of Cultural History, University of Oslo, St. Olavs Plass, P.O. Box 6762, NO-0130, Oslo, Norway.
Björn AnderssonDepartment of Cell and Molecular Biology (CMB), Karolinska Insitutet, P.O. Box 285, 171 77, Stockholm, Sweden.
Andrés AravenaDepartment of Molecular Biology and Genetics, Faculty of Science, Istanbul University, Vezneciler, 34134, Istanbul, Turkey.
Anders GötherströmCentre for Palaeogenetics, Svante Arrhenius Väg 20C, 106 91, Stockholm, Sweden.

Funding

Mersin University BAP project 2019-3-AP3-3729Pioneers of NW Europe The Research Council of Norway project no. 231305The Swedish Research Council project no. 2019-00849
6 · The paper itself

Abstract

Prehistoric chewed pitch has proven to be a useful source of ancient DNA, both from humans and their microbiomes. Here we present the metagenomic analysis of three pieces of chewed pitch from Huseby Klev, Sweden, that were dated to 9,890-9,540 before present. The metagenomic profile exposes a Mesolithic oral microbiome that includes opportunistic oral pathogens. We compared the data with healthy and dysbiotic microbiome datasets and we identified increased abundance of periodontitis-associated microbes. In addition, trained machine learning models predicted dysbiosis with 70-80% probability. Moreover, we identified DNA sequences from eukaryotic species such as red fox, hazelnut, red deer and apple. Our results indicate a case of poor oral health during the Scandinavian Mesolithic, and show that pitch pieces have the potential to provide information on material use, diet and oral health.

Indexed as

MicrobiotaPeriodontitisAnimalsDysbiosisHumansMetagenomeOral Health

Identifiers

PMID38238372
PMCPMC10796427

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.