Assessing protein distribution and dendritic spine morphology relationships using structured illumination microscopy in cultured neurons.

Kathryn J Bjornson, Michael E Cahill

Open access · goldAbstract read

In one paragraph

Article in STAR protocols, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.7field-weighted citation impact, top 23% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

Identification ofProceedings of the National Academy of Sciences of the United States of America · 2025
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Kathryn J BjornsonDepartment of Comparative Biosciences, University of Wisconsin, Madison, WI 53706, USA. Electronic address: kbjornson@wisc.edu.

Michael E CahillDepartment of Comparative Biosciences, University of Wisconsin, Madison, WI 53706, USA. Electronic address: michael.cahill@wisc.edu.

University of Wisconsin–Madison · USUniversity of Wisconsin System · US

Funding

The role of Akt signaling in prefrontal circuit function and cognitive impairmentR21MH125227 · NIMH · UNIVERSITY OF WISCONSIN-MADISON · PI CAHILL, MICHAEL EDWARD · 2021 to 2022

$407k

NIMH NIH HHS R21 MH125227

6 · The paper itself

Abstract

Dendritic spines are protrusions on dendrites forming the postsynaptic aspect of excitatory connections within the brain. Spine morphology is associated with synaptic functional strength and the spatial regulation of protein nanodomains within dendritic spines is an important determinant of spine structure and function. Here, we present a protocol to resolve the nanoscale localization of proteins within dendritic spines using structured illumination microscopy. We describe steps for the structural analysis of dendritic spine parameters, protein localization analysis, and data processing. For complete details on the use and execution of this protocol, please refer to Bjornson et al.

Indexed as

Dendritic SpinesMicroscopyLightingNeuronsCell cultureMicroscopyNeuroscience

Identifiers

PMID38236769

DOI10.1016/j.xpro.2023.102829

PMCPMC10827590

OpenAlexW4390901917

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC-ND

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.