Evidence map›Paper›PMID 38236550›Full record

ArticleMolecular biology reports2024

Assemblage characterization of Giardia duodenalis in South Khorasan province, eastern Iran, using HRM real-time PCR method.

Moloud Tabrizi, Mahmoodreza Behravan, Seyyed Javad Seyyed Tabaei, Zohreh Lasjerdi, Mohammad Amin Pourhoseingholi, Hanieh Mohammad Rahimi, Hamed Mirjalali, Ali Haghighi

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Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1 · What the graph read from it

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Moloud TabriziDepartment of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mahmoodreza BehravanInfectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran.
Seyyed Javad Seyyed TabaeiDepartment of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Zohreh LasjerdiDepartment of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mohammad Amin PourhoseingholiBasic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Hanieh Mohammad RahimiFoodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Hamed MirjalaliFoodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran. hamedmirjalali@sbmu.ac.ir.
Ali HaghighiDepartment of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. a_haghighi@sbmu.ac.ir.

Funding

School of Medicine, Shahid Beheshti University of Medical Sciences 25680
6 · The paper itself

Abstract

backgroundGiardia duodenalis is a common parasitic protozoan causing gastrointestinal illness in humans worldwide. The genetic diversity of G. duodenalis is reflected through the identification of different assemblages. In this study, we aimed to determine the assemblages of G. duodenalis in eastern Iran using nested-PCR and high-resolution melting (HRM) real-time PCR methods.

methodsA total of 58 positive G. duodenalis, which were isolated from 1800 subjects, referred to medical center laboratories in South Khorasan province, eastern Iran, from April 2020 to March 2022, were included in this study. DNA was extracted and HRM real-time PCR was performed for assemblage characterization.

resultsHRM real-time PCR successfully characterized all samples. Accordingly, out of 58 positive samples, 53 (91.36%) and 5 (8.62%) were identified as assemblage A and B, respectively.

conclusionsOur findings showed that HRM real-time PCR was able to characterize the assemblages of G. duodenalis. In addition, our results suggest high prevalence of assemblage A in eastern region of Iran.

Indexed as

Giardia lambliaHospitalsHumansIranLaboratoriesReal-Time Polymerase Chain ReactionAssemblagesGiardia duodenalisHRM real-time PCRIran

Identifiers

PMID38236550

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