Evidence map›Paper›PMID 38236018›Full record

ArticleApplied and environmental microbiology2024

Engineering the thermal stability of a polyphosphate kinase by ancestral sequence reconstruction to expand the temperature boundary for an industrially applicable ATP regeneration system.

Zong-Lin Li, Chuan-Qi Sun, Zhou-Lei Qing, Zhi-Min Li, Hong-Lai Liu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied and environmental microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.9field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 8 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Zong-Lin Li *State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.ORCID 0000-0002-1729-9593
Chuan-Qi Sun *State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Zhou-Lei QingState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Zhi-Min LiState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.ORCID 0000-0002-5746-8275
Hong-Lai LiuSchool of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, China.
East China University of Science and Technology · CN

Funding

MOST | National Key Research and Development Program of China (NKPs) 2022YFC3401700MOST | National Natural Science Foundation of China (NSFC) 32171478Tianjin synthetic biotechnology innovation capacity improvement project TSBICIP-KJGG-009
6 · The paper itself

Abstract

ATP-dependent energy-consuming enzymatic reactions are widely used in cell-free biocatalysis. However, the direct addition of large amounts of expensive ATP can greatly increase cost, and enzymatic production is often difficult to achieve as a result. Although a polyphosphate kinase (PPK)-polyphosphate-based ATP regeneration system has the potential to solve this challenge, the generally poor thermal stability of PPKs limits the widespread use of this method. In this paper, we evaluated the thermal stability of a PPK from

Indexed as

Adenosine TriphosphateEpsilonproteobacteriaPhosphotransferases (Phosphate Group Acceptor)NucleotidesTemperatureAdenosine TriphosphateNucleotidesPhosphotransferases (Phosphate Group Acceptor)polyphosphate kinaseancestral sequence reconstructionATP regenerationmolecular dynamics simulationpolyphosphate kinasethermal stability engineering

Identifiers

PMID38236018
PMCPMC10880597
OpenAlexW4390974009

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.