Evidence map›Paper›PMID 38234765›Full record

ArticleResearch square2023

Sub-Zero Non-Freezing of Vascularized Composite Allografts Preservation in Rodents.

Irina Filz von Reiterdank, Pierre Tawa, Yanis Berkane, Eloi de Clermont-Tonnerre, Antonia Dinicu, Casie Pendexter, Marion Goutard, Alexandre G Lellouch, Aebele B Mink van der Molen, J Henk Coert and 2 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors.

Irina Filz von ReiterdankCenter for Engineering in Medicine and Surgery, Derpartment of Surgery, Massachusetts General Hospital, Harvard Medical School.
Pierre TawaDepartment of Plastic, Reconstructive and Aesthetic Surgery, Hôpital Paris Saint-Joseph.
Yanis BerkaneDepartment of Plastic, Reconstructive and Aesthetic Surgery, Hôpital Sud, CHU Rennes, University of Rennes.
Eloi de Clermont-TonnerreDepartment of Plastic, Reconstructive and Aesthetic Surgery, Hôpital Paris Saint-Joseph.
Antonia DinicuCenter for Engineering in Medicine and Surgery, Derpartment of Surgery, Massachusetts General Hospital, Harvard Medical School.
Casie PendexterCenter for Engineering in Medicine and Surgery, Derpartment of Surgery, Massachusetts General Hospital, Harvard Medical School.
Marion GoutardDepartment of Plastic, Reconstructive and Aesthetic Surgery, Hôpital Paris Saint-Joseph.
Alexandre G LellouchInnovative Therapies in Haemostasis, INSERM UMR-S 1140, University of Paris, F-75006.
Aebele B Mink van der MolenDepartment of Plastic, Reconstructive and Hand Surgery, University Medical Center Utrecht, Utrecht University.
J Henk CoertDepartment of Plastic, Reconstructive and Hand Surgery, University Medical Center Utrecht, Utrecht University.
Curtis L CetruloVascularized Composite Allotransplantation Laboratory, Massachusetts General Hospital, Harvard Medical School.
Korkut UygunCenter for Engineering in Medicine and Surgery, Derpartment of Surgery, Massachusetts General Hospital, Harvard Medical School.

Funding

Genetic-engineered control of the immunogeneic state of vascular composite allografts during preservationR01EB028782 · NIBIB · MASSACHUSETTS GENERAL HOSPITAL · PI Biju Parekkadan, Korkut Uygun · 2021 to 2026
$3.1M
Subzero preservation of vascular composite allograftsR56AI171958 · NIAID · MASSACHUSETTS GENERAL HOSPITAL · PI BISCHOF, JOHN C, FINGER, ERIK BRIAN · 2022 to 2022
$546k
NIAID NIH HHS R56 AI171958NIBIB NIH HHS R01 EB028782
6 · The paper itself

Abstract

Ischemia is a major limiting factor in Vascularized Composite Allotransplantation (VCA) as irreversible muscular injury can occur after as early as 4-6 hours of static cold storage (SCS). Organ preservation technologies have led to the development of storage protocols extending rat liver ex vivo preservation up to 4 days. Development of such a protocol for VCAs has the added challenge of inherent ice nucleating factors of the graft, therefore this study focused on developing a robust protocol for VCA supercooling. Rodent partial hindlimbs underwent subnormothermic machine perfusion (SNMP) with several loading solutions, followed by cryoprotective agent (CPA) cocktail developed for VCAs. Storage occurred in suspended animation for 24h and VCAs were recovered using SNMP with modified Steen. This study shows a robust VCA supercooling preservation protocol in a rodent model. Further optimization is expected to allow for its application in a transplantation model, which would be a breakthrough in the field of VCA preservation.

Identifiers

PMID38234765
PMCPMC10793490

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.