ArticleThe Journal of international medical research2024
A multiplex recombinase polymerase amplification assay combined with CRISPR/Cas12a for the detection of respiratory syncytial virus and respiratory adenovirus.
Article in The Journal of international medical research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The trial behind it
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Who cites it
8 citing papers in PubMed, 10 citations in OpenAlex.
- Review
- Rapid, sensitive, and highly specific diagnosis of respiratory syncytial virus using recombinase polymerase amplification-based biosensor and fluorescence detection.Frontiers in cellular and infection microbiology · 2026Article
- CRISPR-Cas-Based Diagnostics in Biomedicine: Principles, Applications, and Future Trajectories.Biosensors · 2025Review
- Development of a Rapid Isothermal Assay for Detection of Adenovirus Types Important in Respiratory Infections.Influenza and other respiratory viruses · 2025Article
- Detection of KPC-Producing Carbapenem-ResistantJournal of microbiology and biotechnology · 2025Article
- Rapid and sensitive detection of human adenovirus types 3 and 7 using CRISPR-Cas12b coupled with multiple cross displacement amplification.Infectious medicine · 2025Article
- Detection of respiratory syncytial virus based on RT-RPA and CRISPR-Cas12a.Experimental biology and medicine (Maywood, N.J.) · 2025Article
- Respiratory syncytial virus: A review of current basic and clinical knowledge.Qatar medical journal · 2024Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveRespiratory syncytial virus (RSV) and respiratory adenovirus (ADV) are two common pathogens that cause acute respiratory tract infections in children. We aimed to develop a rapid method for detecting both pathogens simultaneously.
methodsThe recombinase polymerase isothermal amplification (RPA) method was combined with the CRISPR/Cas detection system. The assay's specificity and sensitivity were explored by designing RPA primers and CRISPR RNAs (crRNAs) through multi-sequence comparisons, optimizing the reaction conditions, and using a fluorescent reading device. The consistency of the test results of 160 clinical pharyngeal swab samples was studied using quantitative polymerase chain reaction (qPCR) results as a comparative control.
resultsRSV and ADV could be detected at levels as low as 10
conclusionThis method for detecting RSV and ADV is rapid, sensitive, and specific. It can accurately detect mixed infections in a timely manner, making it suitable for use in areas with scarce healthcare resources.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.