Evidence map›Paper›PMID 38229168›Full record

ArticleJournal of biological engineering2024

Stable, neuron-specific gene expression in the mouse brain.

Osama Ahmed, Kingsley M Ekumi, Francesco V Nardi, Gulimiheranmu Maisumu, Khaled Moussawi, Eric D Lazartigues, Bo Liang, Abraam M Yakoub

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biological engineering, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

Osama AhmedDepartment of Medicine, Harvard Medical School, and Brigham and Women's Hospital, Boston, MA, USA.
Kingsley M EkumiBiomedical Engineering Program, University of North Dakota, Grand Forks, ND, USA.
Francesco V NardiDepartment of Medicine, Harvard Medical School, and Brigham and Women's Hospital, Boston, MA, USA.
Gulimiheranmu MaisumuDepartment of Medicine, Harvard Medical School, and Brigham and Women's Hospital, Boston, MA, USA.
Khaled MoussawiDepartment of Psychiatry, University of Pittsburgh, Pittsburgh, PA, USA.
Eric D LazartiguesCardiovascular Center of Excellence, Louisiana State University Health Sciences Center, New Orleans, LA, USA.
Bo LiangBiomedical Engineering Program, University of North Dakota, Grand Forks, ND, USA.
Abraam M YakoubDepartment of Medicine, Harvard Medical School, and Brigham and Women's Hospital, Boston, MA, USA. ayakoub@bwh.harvard.edu.
Brigham and Women's Hospital · USUniversity of North Dakota · USSoutheast Louisiana Veterans Health Care System · USUniversity of Pittsburgh · US

Funding

SARS-CoV-2 tropism in the brain and its relationship to COVID-19 pathogenesisR01HL163814 · NHLBI · UNIVERSITY OF NORTH DAKOTA · PI LAZARTIGUES, ERIC D, TSENG, CHIEN-TE K · 2021 to 2021
$4.3M
Autophagon: an Autophagy-Functionalizing Gene Therapy Tool for Neurodegenerative DiseasesR01AG074899 · NIA · UNIVERSITY OF NORTH DAKOTA · PI Abraam M. Yakoub · 2022 to 2026
$3.4M
NHLBI NIH HHS R01 HL163814NIA NIH HHS R01 AG074899
6 · The paper itself

Abstract

Gene delivery to, and expression in, the mouse brain is important for understanding gene functions in brain development and disease, or testing gene therapies. Here, we describe an approach to express a transgene in the mouse brain in a cell-type-specific manner. We use stereotaxic injection of a transgene-expressing adeno-associated virus into the mouse brain via the intracerebroventricular route. We demonstrate stable and sustained expression of the transgene in neurons of adult mouse brain, using a reporter gene driven by a neuron-specific promoter. This approach represents a rapid, simple, and cost-effective method for global gene expression in the mouse brain, in a cell-type-specific manner, without major surgical interventions. The described method represents a helpful resource for genetically engineering mice to express a therapeutic gene, for gene therapy studies, or to deliver genetic material for genome editing and developing knockout animal models.

Indexed as

BrainBrain diseaseGene deliveryGene expressionGene therapyGenetic engineering

Identifiers

PMID38229168
PMCPMC10790494
OpenAlexW4390911996

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.