Evidence map›Paper›PMID 38229067›Full record

ArticleMicrobial cell factories2024

Proteome profiling of enriched membrane-associated proteins unraveled a novel sophorose and cello-oligosaccharide transporter in Trichoderma reesei.

Karoline Maria Vieira Nogueira, Vanessa Mendes, Karthik Shantharam Kamath, Anusha Cheruku, Letícia Harumi Oshiquiri, Renato Graciano de Paula, Claudia Carraro, Wellington Ramos Pedersoli, Lucas Matheus Soares Pereira, Luiz Carlos Vieira and 6 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial cell factories, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.3field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Applied and environmental microbiology · 2026
    Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 5 institutions in 3 countries.

Karoline Maria Vieira Nogueira *Molecular Biotechnology Laboratory, Department of Biochemistry and Immunology, Ribeirao Preto Medical School (FMRP), University of Sao Paulo, Ribeirao Preto, SP, Brazil.
Vanessa Mendes *Molecular Biotechnology Laboratory, Department of Biochemistry and Immunology, Ribeirao Preto Medical School (FMRP), University of Sao Paulo, Ribeirao Preto, SP, Brazil.
Karthik Shantharam KamathDepartment of Natural Sciences, Macquarie University, Sydney, NSW, Australia.
Anusha CherukuAustralian Proteome Analysis Facility, Macquarie University, Sydney, NSW, Australia.
Letícia Harumi OshiquiriMolecular Biotechnology Laboratory, Department of Biochemistry and Immunology, Ribeirao Preto Medical School (FMRP), University of Sao Paulo, Ribeirao Preto, SP, Brazil.
Renato Graciano de PaulaDepartment of Physiological Sciences, Health Sciences Centre, Federal University of Espirito Santo, Vitoria, ES, 29047-105, Brazil.
Claudia CarraroMolecular Biotechnology Laboratory, Department of Biochemistry and Immunology, Ribeirao Preto Medical School (FMRP), University of Sao Paulo, Ribeirao Preto, SP, Brazil.
Wellington Ramos PedersoliMolecular Biotechnology Laboratory, Department of Biochemistry and Immunology, Ribeirao Preto Medical School (FMRP), University of Sao Paulo, Ribeirao Preto, SP, Brazil.
Lucas Matheus Soares PereiraMolecular Biotechnology Laboratory, Department of Biochemistry and Immunology, Ribeirao Preto Medical School (FMRP), University of Sao Paulo, Ribeirao Preto, SP, Brazil.
Luiz Carlos VieiraDepartment of Molecular and Cell Biology, Ribeirao Preto Medical School (FMRP), University of Sao Paulo, Ribeirao Preto, SP, Brazil.
Andrei Stecca SteindorffUS Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Ardeshir AmirkhaniAustralian Proteome Analysis Facility, Macquarie University, Sydney, NSW, Australia.
Matthew J McKayAustralian Proteome Analysis Facility, Macquarie University, Sydney, NSW, Australia.
Helena NevalainenDepartment of Natural Sciences, Macquarie University, Sydney, NSW, Australia.
Mark P MolloyAustralian Proteome Analysis Facility, Macquarie University, Sydney, NSW, Australia.
Roberto N SilvaMolecular Biotechnology Laboratory, Department of Biochemistry and Immunology, Ribeirao Preto Medical School (FMRP), University of Sao Paulo, Ribeirao Preto, SP, Brazil. rsilva@fmrp.usp.br.
Universidade de São Paulo · BRMacquarie University · AUAustralian Genome Research Facility · AUJoint Genome Institute · USUniversidade Federal do Espírito Santo · BR

Funding

Fundação de Amparo à Pesquisa do Estado de São Paulo 2018/25898-3Fundação de Amparo à Pesquisa do Estado de São Paulo 2019/11655-4
6 · The paper itself

Abstract

backgroundTrichoderma reesei is an organism extensively used in the bioethanol industry, owing to its capability to produce enzymes capable of breaking down holocellulose into simple sugars. The uptake of carbohydrates generated from cellulose breakdown is crucial to induce the signaling cascade that triggers cellulase production. However, the sugar transporters involved in this process in T. reesei remain poorly identified and characterized.

resultsTo address this gap, this study used temporal membrane proteomics analysis to identify five known and nine putative sugar transporters that may be involved in cellulose degradation by T. reesei. Docking analysis pointed out potential ligands for the putative sugar transporter Tr44175. Further functional validation of this transporter was carried out in Saccharomyces cerevisiae. The results showed that Tr44175 transports a variety of sugar molecules, including cellobiose, cellotriose, cellotetraose, and sophorose.

conclusionThis study has unveiled a transporter Tr44175 capable of transporting cellobiose, cellotriose, cellotetraose, and sophorose. Our study represents the first inventory of T. reesei sugar transportome once exposed to cellulose, offering promising potential targets for strain engineering in the context of bioethanol production.

Indexed as

CellulaseGlucansHypocrealesTrichodermaCellobioseCelluloseMembrane ProteinsMembrane Transport ProteinsOligosaccharidesProteomeSaccharomyces cerevisiaeSugarsCellobioseCellulaseCelluloseGlucansMembrane ProteinsMembrane Transport ProteinsOligosaccharidesProteomesophoroseSugarsCelluloseMembrane-associated proteomeSugar transportersTrichoderma reesei

Identifiers

PMID38229067
PMCPMC10790555
OpenAlexW4390903271

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.