Evidence map›Paper›PMID 38227647›Full record

ArticleStem cells (Dayton, Ohio)2024

Ubiquitin E3 Ligase FBXO9 Regulates Pluripotency by Targeting DPPA5 for Ubiquitylation and Degradation.

Samantha A Swenson, Kasidy K Dobish, Hendrik C Peters, C Bea Winship, R Willow Hynes-Smith, Mika Caplan, Karli J Wittorf, Gargi Ghosal, Shannon M Buckley

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells (Dayton, Ohio), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Samantha A SwensonDepartment of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, USA.
Kasidy K DobishFred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE, USA.
Hendrik C PetersHuntsman Cancer Institute, University of Utah, Salt Lake City, UT,USA.
C Bea WinshipHuntsman Cancer Institute, University of Utah, Salt Lake City, UT,USA.
R Willow Hynes-SmithFred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE, USA.
Mika CaplanFred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE, USA.
Karli J WittorfFred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE, USA.
Gargi GhosalFred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE, USA.
Shannon M BuckleyHuntsman Cancer Institute, University of Utah, Salt Lake City, UT,USA.ORCID 0000-0002-0523-2893
University of Nebraska Medical Center · USUniversity of Utah · USNebraska Medical Center · US

Funding

UTAH REGIONAL CANCER CENTERP30CA042014 · NCI · UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH · PI KATHARINE S ULLMAN · 1986 to 2026
$72.6M
UNMC/EPPLEY CANCER CENTER SUPPORT GRANTP30CA036727 · NCI · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI Joann B. Sweasy · 1985 to 2026
$55.0M
Target Validation CoreP20GM121316 · NIGMS · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI Robert E. Lewis · 2018 to 2026
$23.5M
Role of ubiquitin ligases in mantle cell lymphomaR37CA262635 · NCI · UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH · PI Shannon Buckley · 2021 to 2026
$2.4M
Mechanisms underlying USP1-mediated bypass of EWS-FLI1 oncogene-induced replication stress in Ewing sarcomaR01CA263504 · NCI · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI GARGI GHOSAL · 2022 to 2026
$1.7M
Regulation of SPRTN protease and SPRTN-mediated DNA-Protein Crosslink RepairR01GM141232 · NIGMS · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI GHOSAL, GARGI · 2022 to 2025
$1.3M
NCI NIH HHS P30 CA036727NCI NIH HHS P30 CA042014NCI NIH HHS R01 CA263504NCI NIH HHS R37 CA262635NIGMS NIH HHS P20 GM121316NIGMS NIH HHS R01 GM141232
6 · The paper itself

Abstract

Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) have unique characteristics where they can both contribute to all three germ layers in vivo and self-renewal indefinitely in vitro. Post-translational modifications of proteins, particularly by the ubiquitin proteasome system (UPS), control cell pluripotency, self-renewal, and differentiation. A significant number of UPS members (mainly ubiquitin ligases) regulate pluripotency and influence ESC differentiation with key elements of the ESC pluripotency network (including the "master" regulators NANOG and OCT4) being controlled by ubiquitination. To further understand the role of the UPS in pluripotency, we performed an RNAi screen during induction of cellular reprogramming and have identified FBXO9 as a novel regulator of pluripotency associated protein DPPA5. Our findings indicate that FBXO9 silencing facilitates the induction of pluripotency through decreased proteasomal degradation of DPPA5. These findings identify FBXO9 as a key regulator of pluripotency.

Indexed as

Embryonic Stem CellsF-Box ProteinsProteasome Endopeptidase ComplexUbiquitin-Protein LigasesAnimalsCell DifferentiationCellular ReprogrammingMiceProteinsUbiquitinUbiquitinationDppa5 protein, mouseF-Box ProteinsFBXO9 protein, mouseProteasome Endopeptidase ComplexProteinsUbiquitinUbiquitin-Protein LigasesDPPA5E3 ligasepluripotencyubiquitination

Identifiers

PMID38227647
PMCPMC11016844
OpenAlexW4390904531

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.