Evidence map›Paper›PMID 38227062›Full record

ArticleMolecular biology reports2024

GLP-1 signaling-regulated SNAP25 is involved in improved insulin secretion in diabetic GK rats after Roux-en-Y gastric bypass surgery.

Xiangchen Kong, Chao Lin, Chenxi Yang, Xin Wang, Bingfeng Li, Dan Yan, Yanhui Yang, Anyi Hu, Yanyin Chen, Xiaohui Xu and 1 more

Abstract read
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In one paragraph

Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 citations in OpenAlex.

  1. Article
  2. GLP-1 agonists and the gut microbiome: A bidirectional relationship.British journal of clinical pharmacology · 2026
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Xiangchen KongSchool of Medicine, Shenzhen University Diabetes Institute, Shenzhen University, Shenzhen, 518060, China. kxc1981@yeah.net.ORCID http://orcid.org/0000-0001-5149-1987
Chao LinSchool of Medicine, Shenzhen University Diabetes Institute, Shenzhen University, Shenzhen, 518060, China.
Chenxi YangSchool of Medicine, Shenzhen University Diabetes Institute, Shenzhen University, Shenzhen, 518060, China.
Xin WangSchool of Medicine, Shenzhen University Diabetes Institute, Shenzhen University, Shenzhen, 518060, China.
Bingfeng LiSchool of Medicine, Shenzhen University Diabetes Institute, Shenzhen University, Shenzhen, 518060, China.
Dan YanSchool of Medicine, Shenzhen University Diabetes Institute, Shenzhen University, Shenzhen, 518060, China.
Yanhui YangSchool of Medicine, Shenzhen University Diabetes Institute, Shenzhen University, Shenzhen, 518060, China.
Anyi HuSchool of Medicine, Shenzhen University Diabetes Institute, Shenzhen University, Shenzhen, 518060, China.
Yanyin ChenSchool of Medicine, Shenzhen University Diabetes Institute, Shenzhen University, Shenzhen, 518060, China.
Xiaohui XuShenzhen University General Hospital, Shenzhen, 518055, China.
Xiaosong MaSchool of Medicine, Shenzhen University Diabetes Institute, Shenzhen University, Shenzhen, 518060, China.
Shenzhen University · CN

Funding

National Natural Science Foundation of China 81670708, 82070845National Natural Science Foundation of China 82000781National Natural Science Foundation of China 82070806
6 · The paper itself

Abstract

backgroundRoux-en-Y gastric bypass surgery (RYGB) improves glucose-stimulated insulin secretion (GSIS) in type 2 diabetes (T2D) patients. SNAP25 plays an essential role in GSIS. Clinical studies indicate that enhanced GLP-1 signaling is an important contributor to the improved β-cell function in T2D. We aimed to explore whether GLP-1-regulated SNAP25 is involved in the enhanced secretory function of β-cells in diabetic Goto-Kakizaki (GK) rats after RYGB. METHODS AND

resultsRYGB or sham surgery was conducted in GK rats. mRNA and protein expression of SNAP25 was assessed by qPCR and Western blot, respectively. Occupancy of CREB and acetyltransferase CBP and acetylation of histone H3 (ACH3) at the Snap25 promoter were determined using ChIP assay. RYGB led to increased SNAP25 expression and CREB phosphorylation in islets from GK rats. Increased SNAP25 improved GSIS in β-cells cultured in high glucose conditions. Consistent with increased plasma GLP-1 after RYGB, GLP-1R agonist exendin4 increased SNAP25 expression and CREB phosphorylation in β-cells. Mechanistically, exendin4 promoted the recruitment of CREB and CBP, thereby increasing ACH3 at the Snap25 promoter. Consistently, inhibition of CBP attenuated the effect of exendin4 on SNAP25 expression. Furthermore, the knockdown of SNAP25 diminished the increase of GSIS potentiated by chronic GLP-1 culture in INS-1 832/13 cells.

conclusionsOur findings unravel the novel mechanisms of RYGB-enhanced SNAP25 expression in β-cells, and SNAP25 may contribute to the improved β-cell secretory function induced by RYGB.

Indexed as

Diabetes Mellitus, Type 2Gastric BypassInsulin SecretionSynaptosomal-Associated Protein 25AnimalsGlucagon-Like Peptide 1GlucoseHistonesRatsGlucagon-Like Peptide 1GlucoseHistonesSnap25 protein, ratSynaptosomal-Associated Protein 25CREBGLP-1RYGBSNAP25

Identifiers

PMID38227062
OpenAlexW4390907875

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.