Evidence map›Paper›PMID 38225547›Full record

ArticleBMC genomics2024

Transcriptome analysis of long non-coding RNA and mRNA Profiles in VSV-infected BHK-21 Cells.

Wuweiyi Han, Xiaojuan Fei, Fan Yang, Xintong Sun, Jianshe Yang, Jinxin Qiu, Luhua Zhang, Wenhui Zhang, Guohua Chen, Wei Han and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 99% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 1 country.

Wuweiyi Han *Hebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science &Technology, Qinhuangdao, 066004, China.
Xiaojuan Fei *Center of Gansu Provincial Vaccine Engineering Research, Lanzhou Institute of Biological Products, Lanzhou, 730046, Gansu Province, China.
Fan Yang *State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Xintong SunState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Jianshe YangState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Jinxin QiuState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Luhua ZhangState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Wenhui ZhangState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Guohua ChenState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Wei HanShandong Zhuohua Biotechnology Co., Ltd, Liaocheng, 252126, China.
Xiaobo HeShandong Zhuohua Biotechnology Co., Ltd, Liaocheng, 252126, China.
Yongsheng LiuHebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science &Technology, Qinhuangdao, 066004, China. liuys4031@hevttc.edu.cn.
Weike LiState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China. liweike@caas.cn.
Lanzhou Veterinary Research Institute · CNHebei Normal University of Science and Technology · CNLanzhou University · CNChinese Academy of Agricultural Sciences · CNLanzhou Institute of Husbandry and Pharmaceutical Sciences · CN

Funding

Natural Science Foundation of Hebei Province Project Funds, CPXRA Two-component system Regulation mechanism of polymyxin Resistance of Salmonella Typhimurium C2022407019Project Funds supported by Hebei Normal University of Science and Technology, Diagnostic techniques and epidemiology of infectious respiratory diseases in cattle 2022YB009 and 2022YB010Science and Technology Major Project of Gausu Province 22ZD6NA001The National Natural Sciences Foundation of China 32372984The Natural Science Foundation of Gausu Province of China 21JR7RA020the project from a supporting grant of Lanzhou Veterinary Research Institute 1610312021004
6 · The paper itself

Abstract

backgroundVesicular stomatitis virus (VSV) is a typical non-segmented negative-sense RNA virus of the genus Vesiculovirus in the family Rhabdoviridae. VSV can infect a wide range of animals, including humans, with oral blister epithelial lesions. VSV is an excellent model virus with a wide range of applications as a molecular tool, a vaccine vector, and an oncolytic vector. To further understand the interaction between VSV and host cells and to provide a theoretical basis for the application prospects of VSV, we analyzed the expression of host differentially expressed genes (DEGs) during VSV infection using RNA-Seq.

resultsOur analyses found a total of 1015 differentially expressed mRNAs and 161 differentially expressed LncRNAs in BHK-21 cells infected with VSV for 24 h compared with controls. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment showed that the differentially expressed lncRNAs and their target genes were mainly concentrated in pathways related to apoptosis, cancer, disease, and immune system activation, including the TNF, P53, MAPK, and NF-kappaB signaling pathways. The differentially expressed lncRNA can modulate immune processes by regulating genes involved in these signaling transmissions. Ten randomly selected DEGs, namely, Il12rb2, F2, Masp2, Mcl1, FGF18, Ripk1, Fas, BMF, POLK, and JAG1, were validated using RT-qPCR. As predicted through RNA-Seq analysis, these DEGs underwent either up- or downregulation, suggesting that they may play key regulatory roles in the pathways mentioned previously.

conclusionsOur study showed that VSV infection alters the host metabolic network and activates immune-related pathways, such as MAPK and TNF. The above findings provide unique insights for further study of the mechanism of VSV-host interactions and, more importantly, provide a theoretical basis for VSV as an excellent vaccine carrier.

Indexed as

RNA, Long NoncodingVaccinesAnimalsGene Expression ProfilingHumansRNA, MessengerRNA-SeqTranscriptomeRNA, Long NoncodingRNA, MessengerVaccinesDifferentially expressed genesLncRNAmRNARNA-SeqVesicular stomatitis virus

Identifiers

PMID38225547
PMCPMC10789022
OpenAlexW4390871344

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