Evidence map›Paper›PMID 38225505›Full record

ArticleWorld journal of microbiology & biotechnology2024

Identification a novel Ganoderma FIP gene from Ganoderma capense and its functional expression in Pichia pastoris.

Jingwei Lin, Yating Liao, Sijia Yang, Taicheng Jin, Boning Yu, Kai Zhao, Yixiao Sai, Cheng Lin, Yanhua Song, Hui Ma and 1 more

Abstract read
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In one paragraph

Article in World journal of microbiology & biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.3field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. High-Yield-Related Genes Participate in Mushroom Production.Journal of fungi (Basel, Switzerland) · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Jingwei Lin *College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, China.
Yating Liao *College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, China.
Sijia Yang *College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, China.
Taicheng JinSchool of Life Science, Jilin Normal University, Siping, 136000, China.
Boning YuCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, China.
Kai ZhaoCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, China.
Yixiao SaiCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, China.
Cheng LinCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, China.
Yanhua SongCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, China.
Hui MaCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, China. mahui@syau.edu.cn.
Zhanyong WangCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, China. wangzy125@syau.edu.cn.
Shenyang Agricultural University · CNJilin Normal University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ganoderma capense is a precious medicinal fungus in China. In this study, a novel fungal immunomodulatory protein gene, named as FIP-gca, was cloned from G. capense by homologous cloning. Sequencing analysis indicated that FIP-gca was composed of 336 bp, which encoded a polypeptide of 110 amino acids. Protein sequence blasting and phylogenetic analysis showed that FIP-gca shared homology with other Ganoderma FIPs. FIP-gca was effectively expressed in Pichia pastoris GS115 at an expression level of 166.8 mg/L and purified using HisTrap™ fast-flow prepack columns. The immunomodulation capacity of rFIP-gca was demonstrated by that rFIP-gca could obviously stimulate cell proliferation and increase IL-2 secretion of murine spleen lymphocytes. Besides, antitumor activity of rFIP-gca towards human stomach cancer AGS cell line was evaluated in vitro. Cell wound scratch assay proved that rFIP-gca could inhibit migration of AGS cells. And flow cytometry assay revealed that rFIP-gca could significantly induce apoptosis of AGS cells. rFIP-gca was able to induce 18.12% and 22.29% cell apoptosis at 0.3 μM and 0.6 μM, respectively. Conclusively, the novel FIP-gca gene from G. capense has been functionally expressed in Pichia and rFIP-gca exhibited ideal immunomodulation and anti-tumour activities, which implies its potential application and study in future.

Indexed as

GanodermaSaccharomycetalesAnimalsFungal ProteinsHumansMicePhylogenyPichiaFungal ProteinsAnti-tumourFlow cytometryFungal immunomodulatory proteinGanoderma capenseRecombinant expression

Identifiers

PMID38225505
OpenAlexW4390893504

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.