Evidence map›Paper›PMID 38224153›Full record

ArticleCytoskeleton (Hoboken, N.J.)2024

Assembly of FAP93 at the proximal axoneme in Chlamydomonas cilia.

Juyeon Hwang, Haruaki Yanagisawa, Keira C Davis, Emily L Hunter, Laura A Fox, Ariana R Jimenez, Reagan E Goodwin, Sarah A Gordon, Courtney D E Stuart, Raqual Bower and 5 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cytoskeleton (Hoboken, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 6 institutions in 2 countries.

Juyeon HwangDepartment of Cell Biology, Emory University, Atlanta, Georgia, USA.
Haruaki YanagisawaGraduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Keira C DavisDepartment of Cell Biology, Emory University, Atlanta, Georgia, USA.
Emily L HunterDepartment of Cell Biology, Emory University, Atlanta, Georgia, USA.
Laura A FoxDepartment of Cell Biology, Emory University, Atlanta, Georgia, USA.
Ariana R JimenezDivision of Natural Sciences, Oglethorpe University, Atlanta, Georgia, USA.
Reagan E GoodwinDivision of Natural Sciences, Oglethorpe University, Atlanta, Georgia, USA.
Sarah A GordonDivision of Natural Sciences, Oglethorpe University, Atlanta, Georgia, USA.
Courtney D E StuartDivision of Natural Sciences, Oglethorpe University, Atlanta, Georgia, USA.
Raqual BowerDepartment of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, USA.
Mary E PorterDepartment of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, USA.
Susan K DutcherDepartment of Genetics, Washington University St. Louis, St. Louis, Missouri, USA.
Winfield S SaleDepartment of Cell Biology, Emory University, Atlanta, Georgia, USA.ORCID https://orcid.org/0000-0003-4966-2120
Karl F LechtreckDepartment of Cellular Biology, University of Georgia, Athens, Georgia, USA.ORCID https://orcid.org/0000-0002-6219-6470
Lea M AlfordDivision of Natural Sciences, Oglethorpe University, Atlanta, Georgia, USA.ORCID https://orcid.org/0000-0002-6334-9213
Emory University · USOglethorpe University · USUniversity of Minnesota · USThe University of Tokyo · JPUniversity of Georgia · USWashington University in St. Louis · US

Funding

Assembly, Targeting, And Regulation Of Dynein MotorsR01GM055667 · NIGMS · UNIVERSITY OF MINNESOTA TWIN CITIES · PI PORTER, MARY E. · 1997 to 2023
$5.4M
Assembly , Targeting, and Regulation of Dynein MotorsR37GM055667 · NIGMS · UNIVERSITY OF MINNESOTA TWIN CITIES · PI PORTER, MARY E. · 2005 to 2014
$4.0M
Functional Substructure of Flagellar DyneinR37GM051173 · NIGMS · EMORY UNIVERSITY · PI SALE, WINFIELD S · 2002 to 2011
$4.0M
FUNCTIONAL SUBSTRUCTURE OF FLAGELLAR DYNEINR01GM051173 · NIGMS · EMORY UNIVERSITY · PI SALE, WINFIELD S · 1994 to 2020
$3.6M
Genetic Analysis of Centrioles and CiliaR35GM131909 · NIGMS · WASHINGTON UNIVERSITY · PI SUSAN K DUTCHER · 2019 to 2026
$3.0M
Regulation of protein transport in ciliaR01GM110413 · NIGMS · UNIVERSITY OF GEORGIA · PI LECHTRECK, KARL · 2014 to 2022
$2.7M
Children's Discovery InstituteNational Science FoundationNIGMS NIH HHS R01 GM051173NIGMS NIH HHS R01 GM055667NIGMS NIH HHS R01 GM110413NIGMS NIH HHS R35 GM131909NIGMS NIH HHS R37 GM051173NIGMS NIH HHS R37 GM055667NIH HHSUniversity of Minnesota
6 · The paper itself

Abstract

To identify proteins specific to the proximal ciliary axoneme, we used iTRAQ to compare short (~2 μm) and full-length (~11 μm) axonemes of Chlamydomonas. Known components of the proximal axoneme such as minor dynein heavy chains and LF5 kinase as well as the ciliary tip proteins FAP256 (CEP104) and EB1 were enriched in short axonemes whereas proteins present along the length of the axoneme were of similar abundance in both samples. The iTRAQ analysis revealed that FAP93, a protein of unknown function, and protein phosphatase 2A (PP2A) are enriched in the short axonemes. Consistently, immunoblots show enrichment of FAP93 and PP2A in short axonemes and immunofluorescence confirms the localization of FAP93 and enrichment of PP2A at the proximal axoneme. Ciliary regeneration reveals that FAP93 assembles continuously but more slowly than other axonemal structures and terminates at 1.03 μm in steady-state axonemes. The length of FAP93 assembly correlates with ciliary length, demonstrating ciliary length-dependent assembly of FAP93. Dikaryon rescue experiments show that FAP93 can assemble independently of IFT transport. In addition, FRAP analysis of GFP-tagged FAP93 demonstrates that FAP93 is stably anchored in the axoneme. FAP93 may function as a scaffold for assembly of other specific proteins at the proximal axoneme.

Indexed as

AxonemeChlamydomonasCiliaProtein Phosphatase 2Protein Phosphatase 2axonemeChlamydomonasciliaFAP93

Identifiers

PMID38224153
PMCPMC12239100
OpenAlexW4390885331

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.