Evidence map›Paper›PMID 38218927›Full record

ArticleJournal of orthopaedic surgery and research2024

Inhibition of LINC00958 hinders the progression of osteoarthritis through regulation of the miR-214-3p/FOXM1 axis.

Yingchuan Yin, Qiaojuan He, Jing He, Ying Feng, Yunyun Xu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of orthopaedic surgery and research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 3 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Yingchuan Yin *Endocrinology Department, The Third People's Hospital of Hefei, No.204, Wangjiangdong Road, Hefei, 230022, China. nfmyyc866@163.com.
Qiaojuan He *Endocrinology Department, The Third People's Hospital of Hefei, No.204, Wangjiangdong Road, Hefei, 230022, China.
Jing HeEndocrinology Department, The Third People's Hospital of Hefei, No.204, Wangjiangdong Road, Hefei, 230022, China.
Ying FengEndocrinology Department, The Third People's Hospital of Hefei, No.204, Wangjiangdong Road, Hefei, 230022, China.
Yunyun XuEndocrinology Department, The Third People's Hospital of Hefei, No.204, Wangjiangdong Road, Hefei, 230022, China.
Third People's Hospital of Hefei · CN

Funding

Applied Medicine of Hefei Municipal Health Commission Hwk2021yb013the key project of the Third People's Hospital of Hefei SYKZ2020001
6 · The paper itself

Abstract

objectiveWe investigated the impact of the long noncoding RNA LINC00958 on cellular activity and oxidative stress in osteoarthritis (OA).

methodsWe performed bioinformatics analysis via StarBase and luciferase reporter assays to predict and validate the interactions between LINC00958 and miR-214-3p and between miR-214-3p and FOXM1. The expression levels of LINC00958, miR-214-3p, and FOXM1 were measured by qRT-PCR and western blotting. To assess effects on CHON-001 cells, we performed MTT proliferation assays, evaluated cytotoxicity with a lactate dehydrogenase (LDH) assay, and examined apoptosis through flow cytometry. Additionally, we measured the levels of apoptosis-related proteins, including BAX and BCL2, using western blotting. The secretion of inflammatory cytokines (IL-6, IL-8, and TNF-α) was measured using ELISA.

resultsOur findings confirmed that LINC00958 is a direct target of miR-214-3p. LINC00958 expression was upregulated but miR-214-3p expression was downregulated in both OA cells and IL-1β-stimulated CHON-001 cells compared to the corresponding control cells. Remarkably, miR-214-3p expression was further reduced after miR-214-3p inhibitor treatment but increased following LINC00958-siRNA stimulation. Silencing LINC00958 significantly decreased its expression, and this effect was reversed by miR-214-3p inhibitor treatment. Notably, LINC00958-siRNA transfection alleviated the IL-1β-induced inflammatory response, as evidenced by the increased cell viability, reduced LDH release, suppression of apoptosis, downregulated BAX expression, and elevated BCL2 levels. Moreover, LINC00958 silencing led to reduced secretion of inflammatory factors from IL-1β-stimulated CHON-001 cells. The opposite results were observed in the miR-214-3p inhibitor-transfected groups. Furthermore, in CHON-001 cells, miR-214-3p directly targeted FOXM1 and negatively regulated its expression.

conclusionOur findings suggest that downregulating LINC00958 mitigates IL-1β-induced injury in CHON-001 cells through the miR-214-3p/FOXM1 axis. These results imply that LINC00958 plays a role in OA development and may be a valuable therapeutic target for OA.

Indexed as

MicroRNAsOsteoarthritisApoptosisbcl-2-Associated X ProteinChondrocytesForkhead Box Protein M1HumansInterleukin-1betaRNA, Small Interferingbcl-2-Associated X ProteinForkhead Box Protein M1FOXM1 protein, humanInterleukin-1betaMicroRNAsMIRN214 microRNA, humanRNA, Small InterferingInflammationLINC00958miR-214-3p/FOXM1 axisOsteoarthritis

Identifiers

PMID38218927
PMCPMC10788018
OpenAlexW4390841237

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.