Evidence map›Paper›PMID 38218813›Full record

ArticleBMC veterinary research2024

Molecular and functional characterization of porcine poly C binding protein 1 (PCBP1).

Yue Song, Linqing Wang, Menglong Xu, Xiuxiang Lu, Yumin Wang, Limeng Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Yue Song *Molecule Biology Laboratory of Zhengzhou Normal University, Zhengzhou Henan, 450044, China.
Linqing Wang *Molecule Biology Laboratory of Zhengzhou Normal University, Zhengzhou Henan, 450044, China. wanglintsing@126.com.
Menglong XuCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, 450046, China.
Xiuxiang LuCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, 450046, China.
Yumin WangCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, 450046, China.
Limeng ZhangMolecule Biology Laboratory of Zhengzhou Normal University, Zhengzhou Henan, 450044, China.
Henan Agricultural University · CNZhengzhou Normal University · CN

Funding

Natural Science Foundation of Henan Province 222300420586the Key Scientific Research Projects of Henan Education Department 23B230008
6 · The paper itself

Abstract

backgroundPoly C Binding Protein 1 (PCBP1) belongs to the heterogeneous nuclear ribonucleoprotein family. It is a multifunctional protein that participates in several functional circuits and plays a variety of roles in cellular processes. Although PCBP1 has been identified in several mammals, its function in porcine was unclear.

resultsIn this study, we cloned the gene of porcine PCBP1 and analyzed its evolutionary relationships among different species. We found porcine PCBP1 protein sequence was similar to that of other animals. The subcellular localization of PCBP1 in porcine kidney cells 15 (PK-15) cells was analyzed by immunofluorescence assay (IFA) and revealed that PCBP1 was mainly localized to the nucleus. Reverse transcription-quantitative PCR (RT-qPCR) was used to compare PCBP1 mRNA levels in different tissues of 30-day-old pigs. Results indicated that PCBP1 was expressed in various tissues and was most abundant in the liver. Finally, the effects of PCBP1 on cell cycle and apoptosis were investigated following its overexpression or knockdown in PK-15 cells. The findings demonstrated that PCBP1 knockdown arrested cell cycle in G0/G1 phase, and enhanced cell apoptosis.

conclusionsPorcine PCBP1 is a highly conserved protein, plays an important role in determining cell fate, and its functions need further study.

Indexed as

Carrier ProteinsRNA-Binding ProteinsAnimalsApoptosisDNA-Binding ProteinsHeterogeneous-Nuclear RibonucleoproteinsMammalsProtein BindingSwineCarrier ProteinsDNA-Binding ProteinsHeterogeneous-Nuclear RibonucleoproteinsRNA-Binding ProteinsApoptosisCell cyclesPorcine poly C binding protein 1Subcellular

Identifiers

PMID38218813
PMCPMC10787444
OpenAlexW4390839737

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.