ArticleLight, science & applications2024
Observation of a single protein by ultrafast X-ray diffraction.
Article in Light, science & applications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 30 citations in OpenAlex.
- Model-free pattern separation of two-color ultrafast X-ray diffraction.Nature communications · 2026Article
- Improved image reconstruction in coherent diffraction imaging using self-seeded XFEL pulses.Journal of synchrotron radiation · 2026Article
- Dichography: two-frame ultrafast imaging from a single diffraction pattern.Nature communications · 2026Article
- High-throughput in situ single particle X-ray imaging of dehydrating viral capsids.Light, science & applications · 2026Article
- Single-Particle X-ray Scattering Reveals a High Local Supersaturation of Precursors as the Origin of CoO Assembly Formation.The journal of physical chemistry letters · 2026Article
- Native mass spectrometry meets X-rays for the elucidation of protein structures.Biochemical Society transactions · 2026Review
- Biological applications at the AQUA beamline of the EuPRAXIA@SPARC_LAB free electron laser.European biophysics journal : EBJ · 2026Article
- Partial orientation retrieval of proteins from X-ray free-electron-laser induced explosions for single particle imaging.Scientific reports · 2025Article
- Impact of gas background on XFEL single-particle imaging.Scientific reports · 2025Article
- Modeling electron dynamics in silicon driven by high-intensity femtosecond x-rays.Structural dynamics (Melville, N.Y.) · 2025Article
- Advancing time-resolved structural biology: latest strategies in cryo-EM and X-ray crystallography.Nature methods · 2025Review
- Coaxial helium electrospray for single-particle imaging at X-ray free electron lasers.Journal of synchrotron radiation · 2025Article
- Macromolecular crystallography and biology at the Linac Coherent Light Source.Journal of synchrotron radiation · 2025Article
- Ultrafast spectroscopy of liquids using extreme-ultraviolet to soft-X-ray pulses.Nature reviews. Chemistry · 2025Review
- SPRING, an effective and reliable framework for image reconstruction in single-particle Coherent Diffraction Imaging.npj computational materials · 2025Article
- Aerosol size determination via light scattering of viruses and protein complexes.Communications physics · 2025Article
- Coherent X-ray diffraction imaging of single particles: background impact on 3D reconstruction.Journal of applied crystallography · 2024Article
- High-Performance Molecular Dynamics Simulations for Native Mass Spectrometry of Large Protein Complexes with the Fast Multipole Method.Analytical chemistry · 2024Article
- Article
- Conditional guided generative diffusion for particle accelerator beam diagnostics.Scientific reports · 2024Article
Corrections and comments
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Authors and funding
45 authors at 13 institutions in 8 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The idea of using ultrashort X-ray pulses to obtain images of single proteins frozen in time has fascinated and inspired many. It was one of the arguments for building X-ray free-electron lasers. According to theory, the extremely intense pulses provide sufficient signal to dispense with using crystals as an amplifier, and the ultrashort pulse duration permits capturing the diffraction data before the sample inevitably explodes. This was first demonstrated on biological samples a decade ago on the giant mimivirus. Since then, a large collaboration has been pushing the limit of the smallest sample that can be imaged. The ability to capture snapshots on the timescale of atomic vibrations, while keeping the sample at room temperature, may allow probing the entire conformational phase space of macromolecules. Here we show the first observation of an X-ray diffraction pattern from a single protein, that of Escherichia coli GroEL which at 14 nm in diameter is the smallest biological sample ever imaged by X-rays, and demonstrate that the concept of diffraction before destruction extends to single proteins. From the pattern, it is possible to determine the approximate orientation of the protein. Our experiment demonstrates the feasibility of ultrafast imaging of single proteins, opening the way to single-molecule time-resolved studies on the femtosecond timescale.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.